| 干燥保存人角膜与新鲜角膜内皮细胞的形态学比较 |
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| 引用本文： | 张廷铖 刘健. 干燥保存人角膜与新鲜角膜内皮细胞的形态学比较[J]. 医学争鸣, 1993, 14(1): 63-66 |
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| 作者姓名： | 张廷铖 刘健 |
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| 作者单位： | 西京医院眼科(张廷铖，于纯智，马吉献，彭秀军)，第四军医大学电镜室(刘健，魏正乾，谷法有，李学荣)，第四军医大学电镜室(杨传红) |
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| 摘 要： |
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| 关 键 词： | 角膜 干燥法 内皮细胞 角膜移植 |
The morphological comparison between human endothelial cells of desiccation-preserved and fresh corneas |
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| ZHANG Ting-Yue,YU Chun-Zhi,MA Ji-Xian,PENG Xiu-Jun,LIU Jiun WEI Zheng-Qian,GU Fa-You,LI Xue-Rong and YANG Chucm-Hong. The morphological comparison between human endothelial cells of desiccation-preserved and fresh corneas[J]. Negative, 1993, 14(1): 63-66 |
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| Authors: | ZHANG Ting-Yue YU Chun-Zhi MA Ji-Xian PENG Xiu-Jun LIU Jiun WEI Zheng-Qian GU Fa-You LI Xue-Rong YANG Chucm-Hong |
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| Affiliation: | ZHANG Ting-Yue,YU Chun-Zhi,MA Ji-Xian,PENG Xiu-Jun,LIU Jiun WEI Zheng-Qian,GU Fa-You,LI Xue-Rong and YANG Chucm-Hong Department of Ophthalmology,Xijing Hospital Laboratory of Electron Microscopy |
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| Abstract: | After combined stained with alizarin red S and trypan blue, the endothelial cells of desiccated corneas preserved for 77 days showed that the cellular circumference was blurred and the hexagonal borders could faintly be visible and the mode of distribution of round or elliptical nuclei with blue discoloration was essentially the same as that of fresh normal cornea. Under electron microscope, the cellular outline could be seen with abundant and distinct organellae within the cytoplasm and essentially complete membrane; but the nuclear substance were pale and partially pyknotic. It is suggested that the endothelial cells of cornea preserved by dehydration and desiccation still has vital signs. |
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| Keywords: | cornea desiccation corneal endothelial cell morphology nucleus |
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