The human papillomavirus type 11 E1/\E4 protein is not essential for viral genome amplification |
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Authors: | Fang L Budgeon L R Doorbar J Briggs E R Howett M K |
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Institution: | Department of Bioscience and Biotechnology, Drexel University, Stratton Hall Rm. 118, 3141 Chestnut Street, Philadelphia, PA 19104, USA. |
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Abstract: | An abundant human papillomavirus (HPV) protein E1/\E4 is expressed late in the virus life cycle in the terminally differentiated layers of epithelia. The expression of E1/\E4 usually coincides with the onset of viral DNA amplification. However, the function of E1/\E4 in viral life cycle is not completely understood. To examine the role of E1/\E4 in the virus life cycle, we introduced a single nucleotide change in the HPV-11 genome to result in a truncation of E1/\E4 protein without affecting the E2 amino acid sequence. This mutated HPV-11 genome was introduced into a human foreskin keratinocyte cell line immortalized by the catalytic subunit of human telomerase, deficient in p16(INK4a) expression, and previously shown to support the HPV-11 life cycle when grown in organotypic raft culture. We have demonstrated that E1/\E4 is dispensable for HPV-11 viral DNA amplification in the late stages of the viral life cycle. |
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Keywords: | Human papillomavirus type 11 Organotypic raft culture E1^E4 |
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