吲哚氰绿测定和肝纤维化定量评估肝储备功能的研究

目的：探讨吲哚氰绿和肝纤维化定量测定对评价肝储备功能的价值。方法：制备大鼠肝硬化模型，测定吲哚氰绿15 min潴留率(R15ICG)并使用计算机辅助数字图像分析法检测肝纤维化程度。分析R15ICG与肝纤维化定量评估间的关系，以及两者与肝功能测定间的关系。结果：R15ICG和肝纤维化两指标在对照组、造模8周组和10周组之间差异有统计学意义（P＜0.01）。R15ICG和Child-Pugh评分系统与肝纤维化百分比之间呈直线正相关（r=0.75，0.533；P＜0.05）。结论：吲哚氰绿和肝纤维化定量评估肝功能是较好的指标;两者联合使用有助于全面评价肝脏功能状态。

Evaluaton of liver functional reserve by quantitative assessment of hepatic fibrosis and indocyanine green measurement

Objective:To investigate the value of quantitative assessment of hepatic fibrosis and indocyanine green measurement in predicting hepatic functional reserve in rats with liver cirrhosis. Methods :Female Wister rats were divided into normal control and cirrhotic group,and cirrhotic models were established. Quantitative assessment of hepatic fibrosis was done by use of computer-assisted digital image analysis, and R15ICG was measured. Results:Differences of R15ICG and quantitative assessment of hepatic fibrosis between the two groups at 8 and 10 weers after hepatic fibrosis model was established were statistically significant (P＜0.01). Quantitative assessment of hepatic fibrosis had a positive correlation（r=0.75 and 0.533，P＜0.05）with R15ICG and Child-Pugh score. Conclusions:Combining quantitative assessment of hepatic fibrosis with indocyanine green measurement is helpful for total assessment of liver functional reserve.