| 慢性乙型肝炎血清HBV DNA定量检测及其临床意义 |
| |
| 引用本文： | 陈瑞烈,方婵英,张惠娟,方少鹏.慢性乙型肝炎血清HBV DNA定量检测及其临床意义[J].中国基层医药,2001,8(4):317-318. |
| |
| 作者姓名： | 陈瑞烈 方婵英 张惠娟 方少鹏 |
| |
| 作者单位： | 广东汕头市第二人民医院传染科,515011 |
| |
| 摘 要： | 目的 探讨慢性乙型肝炎（CHB）病人血清HBV DNA含量与HBeAg及肝损害程度的关系。方法 应用定量PCR法和ELISA法检测116例CHB病人血清HBV DNA含量与HBVM。结果 血清HBeAg阳性组HBV DNA含量显著高于HBeAg阴性组（P＜0．05）；CHB轻、中、重度血清HBV DNA含量逐渐降低（P＜0．05）。结论 基因水平证实HBeAg是HBV复制指标；随着肝损害程度加重，血清HBV DNA含量逐渐下降；定量检测HBV DNA对判断肝损害程度、指导抗病毒治疗有重要意义。
|
| 关 键 词： | 慢性乙型肝炎 HBV DNA 定量PCR e抗原 |
| 修稿时间： | 2000年10月12 |
Quantitative detection of HBV DNA in serum of patients with chronic hepatitis B |
| |
| CHEN Ruilie,FANG Chanying,ZHANG Huijuan,et al..Quantitative detection of HBV DNA in serum of patients with chronic hepatitis B[J].Chinese Journal of Primary Medicine and Pharmacy,2001,8(4):317-318. |
| |
| Authors: | CHEN Ruilie FANG Chanying ZHANG Huijuan |
| |
| Institution: | CHEN Ruilie,FANG Chanying,ZHANG Huijuan,et al.Dept. of Infectious Disease of the Second Hospital,Shantou,Guangdong,515011 |
| |
| Abstract: | Objective To investigate the relationship between the degree of liver damage and the level of serum HBV DNA in patients with chronic hepatitis B(CHB).Methods Using quantitative PCR and ELISA to detect serum HBV DNA and HBV marker in 116 patients with CHB respectively.Results The level of serum of HBV DNA was markedly higher in HBeAg positive group than in HBeAg negative group(P<0.05),and was the highest in slight CHB group,the lowest in severe CHB(P<0.05).Conclusion The level of serum HBV DNA verified HBeAg is the marker of HBV replication.Along with aggravation of liver damage,the level of serum HBV DNA decreases.Quantitative detection of serum HBV DNA is useful in assessment of liver damage and guidance in antivirus therapy. |
| |
| Keywords: | Chronic hepatitis B HBV DNA Quantitative PCR E antigen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
