湖北省1999年急性弛缓性麻痹病例实验室监测分析

湖北省 1999年共收检急性弛缓性麻痹 (AFP)病例粪便标本 2 0 8例 ;脊髓灰质炎 (脊灰 )病毒 (PV)检出率为 6 2 5 % ,较往年显著上升 ;非脊灰肠道病毒 (NPEV)检出率 3 37% ,较往年显著下降 ;肠道病毒 (EV)总检出率显著下降。分析原因认为 ,PV检出率的上升可能与麻痹后突击服疫苗再采集粪便标本 ,以及环境接触染毒等因素有关。以L2 0B细胞置换Hep -2是EV总阳性率显著下降的主要原因 ,也是NPEV检出率下降的重要原因。NPEV检出率下降还可能与多种因素 ,如细胞形态、毒性反应及粪便标本的贮运温度等有关

Analysis on the Laboratory Surveillance of Acute Flaccid Paralysis Cases in Hubei Province in 1999

We had collected and tested 208 fecal samples of Acute Flaccid Paralysis (AFP) cases in Hubei Province in 1999 The poliovirus (PV) isolation rate was 6 25%,the non-polio enterovirus (NPEV) isolation rate was 3.37% Compared with the years before, the PV isolation rate increased obviously, meanwhile, both the NPEV isolation rate and the total enterovirus(EV) positive rate decreased significantly We considered that there were two factors causing PV isolation rate increased Firstly, the sample collection was next to OPV emergent vaccination where the paralysis presented;secondly, patients were infected by polio virus existing in the environment beyond national immunization days(NIDs) L20B cells instead of Hep 2 cells was the main reason for decreasing of NPEV and total EV positive isolation rates but this also concerned to the cell growing status,toxic effect of fecal samples and the storage temperatures of the collected samples and sample transportation The reported NPEV isolation rate may be higher than the actual situation in the past few years in our province