Nociceptin inhibits vanilloid TRPV-1-mediated neurosensitization induced by fenoterol in human isolated bronchi |
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Authors: | Faisy Christophe Naline Emmanuel Rouget Céline Risse Paul-André Guerot Emmanuel Fagon Jean-Yves Chinet Thierry Roche Nicolas Advenier Charles |
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Affiliation: | (1) Unité de Recherche de lEnseignement Supérieur, Equipe dAccueil 220, Faculté de Médecine Paris-Ile de France-Ouest, Unité de Formation et de Recherche Biomédicale des Saint-Pères, 45 rue des Saint-Pères, 75006 Paris, France;(2) Service de Réanimation Médicale, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75908 Paris Cedex 15, France |
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Abstract: | Chronic exposure to 2-adrenoceptor agonists, especially fenoterol, has been shown to increase smooth muscle contraction to endothelin-1 in human bronchi partly through tachykinin-mediated pathways. The purpose of this work was to further investigate the role of sensory nerves in fenoterol-induced sensitization of human airways and the effect of nociceptin, a nociceptin/orphanin FQ (NOP) receptor agonist, on the increase in contraction after fenoterol exposure. Human bronchi from 62 patients were sensitized to endothelin-1 by prolonged incubation with fenoterol (0.1 M, 15 h). The sensitizing effect of fenoterol was inhibited by high concentration of capsaicin (10 M, 30 min before fenoterol sensitization), which induces depletion of mediators from sensory nerves, or co-incubation of fenoterol and capsazepine (1 M), a vanilloid TRPV-1 receptor antagonist. Moreover, short pretreatment of bronchi with capsaicin (10 M) or capsazepine (1 M) after sensitization by fenoterol decreased the rise in smooth muscle contraction to endothelin-1. Nociceptin (1 M) also inhibited the increased contraction in fenoterol-sensitized bronchi. Tertiapin (10 M), an inhibitor of the inward-rectifier K+ channels, but not naloxone (0.1 M), a DOP/KOP/MOP receptor antagonist, prevented the inhibitory effect of nociceptin. In conclusion, fenoterol induces sensitization of human isolated bronchi to endothelin-1 in part through the stimulation of the vanilloid TRPV-1 receptor on tachykininergic sensory nerves. Nociceptin inhibits airway hyperresponsiveness via NOP receptor activation. This effect involves inward-rectifier K+ channels. |
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Keywords: | Tachykinins Nociceptin Vanilloid TRPV-1 receptor /content/t2wwrl0ehgpg69lr/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >2-Adrenoceptor Airway hyperresponsiveness Asthma |
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