肝气郁结证勃起功能障碍小鼠模型建立的实验研究

目的:建立肝气郁结证小鼠勃起功能障碍(ED)模型,检测小鼠性器官的变化,揭示ED发生的可能机制。方法:昆明种小鼠,雄性24只,雌性48只,雌雄小鼠都均分为正常组、肝郁组、肝郁加强组。采用束缚盒慢性应激法,逐渐增加束缚强度与时间,造成小鼠肝郁,再阻止雄鼠舔嗅及爬背,压抑其性活动,诱发加强肝气郁结证ED模型。结果:小鼠自主行为、性活动观察及体重均表现出明显的差异性。性器官脏器指数有递减趋势,其病理切片显示正常对照组优于肝郁组及肝郁加强组。结论:束缚盒慢性应激法可造成小鼠肝气郁结证ED,加强因素可增强ED的程度。

Experimental Research on the Liver-qi Stasis Induced Erectile Dysftmction by Mouse Model

Objective: To establish a mouse model of erectile dysfunction(ED) induced by liver-qi stasis for observing the changes in the mouse's sex organs and investigating the possible mechanism of ED. Methods: Seventy-two Kunming mice,24 males and 48 females,were randomly divided into a liver-qi stasis group,a severe liver-qi stasis group and a control group.A stressing box was used to exert gradual stress on the mice of the two experimental groups by confining them to the box and gradually reducing their room of movement so as to induce liver-qi stasis.In addition,the males of the severe liver-qi stasis group were further prohibited from any sexual activities to repress their sexual desire and intensify their liver-qi stasis.Results: Obvious differences were observed in the autonomous behavior,sexual activity and body weight of the mice of the three groups.Pathological examination showed that the sex organ indexes descended in the liver-qi stasis group and even further in the severe liver-qi stasis group as compared with the controls.Conclusion: The stressing box can induce and intensify ED associated with liver-qi stasis.