波形蛋白在增生性瘢痕中表达与作用的研究

目的 波形蛋白是中等纤维的主要成分之一，在细胞骨架与运动中起着重要的作用。探讨波形蛋白在增生性瘢痕中表达程度及对瘢痕增生与挛缩的作用。方法 收集患者不同时期增生与非增生瘢痕作为研究标本，利用基因芯片筛选出的差异基因，选择波形蛋白的基因特异片段，制成寡核苷酸探针与瘢痕组织切片原位杂交。同时，将各标本进行组织块培养，制成成纤维细胞爬片，进行原位杂交。结果 波形蛋白基因在3个月，6个月的增生性瘢痕中表达均明显强于9个月，12个月增生瘢痕及非增生瘢痕，阳性细胞比例也高于9个月，12个月增生瘢痕及非增生瘢痕。结论 瘢痕增生挛缩与细胞骨架运动的相关基因存在密切关系，而波形蛋白起到关键的作用。同时，抑制该基因的表达，可能实现减轻瘢痕增生与挛缩。

Effect of vimentin expression on contraction of hypertrophic scar

Objective Vimentin is one of the proteins of cytoskele to n and cell movement. To investigate the effect of gene expression of fibroblast vimentin on the formation and contraction of hypertrophic scar in this report. Methods According to the results of differently expressed genes in hypertrophic scar by gene microarray, Vimentin, one of the most important g enes , was selected and made into oligonucleotide probe. 24 cases of hypertrophi c scar and 24 non-hypertrophic scar and 12 normal skin were used and these scar were taken on 3, 6, 9 and 12 months after burned. Frozen section and cultured f ibroblasts were made to detect the expression of the gene by in situ hybridizati on. Results Expression of vimentin was defected in scar tissue, but those in the hypertrophic scar on 3 and 6 months after burn were significan tly stronger than those in the hypertrophic scar on 9 and 12 months after burn a nd non-hypertrophic scar. Conclusions Over-expression of cyto skeletal relative genes causes the contraction of scar and vimentin acts leading and key functions.