局灶性脑缺血再灌注大鼠大脑皮质中Caspase-3蛋白的表达

目的：观察局灶性脑缺血再灌注（FCIR）大鼠大脑皮质Caspase-3的表达。方法：雄性SD大鼠42只，分为假手术组和缺血再灌注（IR）组，IR组又分为缺血2h再灌注2h、6h、12h、24h、48h、72h组，每组6只。IR组采用线栓法建立FCIR模型，假手术组仅插线1cm；于脑视交叉平面向后7～11mm处取脑组织。HE染色观察各个时间点细胞形态学变化，免疫组织化学SP法观察不同时间Caspase-3的表达，TUNEL法检测细胞凋亡。结果：脑缺血2h再灌注6hCaspase-3表达升高，再灌注24h达到高峰，再灌注72h开始下降。凋亡细胞数的变化与Caspase-3表达变化一致。结论：Caspase-3参与了脑缺血再灌注神经元的死亡过程，该作用可能与细胞凋亡有关。

Expression of Caspase-3 in rat brain cortex after focal cerebral ischemia and reperfusion

Aim: To observe the expression of Caspase-3 after focal cerebral ischemia and reperfusion in rats brain cortex.Methods:The focal cerebral ischemia and reperfusion model was prepared by thread embolism of middle cerebral artery (MCA). The neuron morphologic change was studied by HE staining. The expression of Caspase-3 in ischemia cortex was determined using immunohistochemical technique. Cell apoptosis was detected using TUNEL.Results: In the process of reperfusion after 2 h ischemia, the expression of Caspase-3 began to increase at 6th h,reached peak at 24th h,and decreased at 72nd h.The change of apoptotic cells was accordant with that of Caspase-3. Conclusion: Caspase-3 regulates the cell apoptosis,which plays a vital role in the injury of rat ischemia and reperfusion.