胃癌及癌前病变粘膜中呈高表达6个基因片段的筛选及其临床意义

目的　筛选数个在胃癌及癌前病变粘膜中检出率高的差异表达基因片段并探讨其临床意义．方法　以筛选出的ｇｃｙｓ１ 至ｇｃｙｓ１８ 的新基因片段作为研究对象，采用定量ＲＴＰＣＲ 技术检测４８ 例胃癌、１６ 例其他肿瘤及１１０ 例活检的胃粘膜标本．结果　①在４８ 例胃癌中，表达检出率高的６ 个片段依次为：ｇｃｙｓ１０ 为９０－５ ％ ，ｇｃｙｓ１ 为８０－９ ％ ，ｇｃｙｓ１８ 为６９－５ ％ ，ｇｃｙｓ１１为６１－９ ％ ，ｇｃｙｓ４ 为４２－８ ％ ，ｇｃｙｓ８ 为３５－７ ％ ，在癌旁组织中检出率＜ １０ ％ ；６ 个片段的检出率在胃癌及癌旁组织中存在显著性差异（ Ｐ ＜ ０－０１） ；②在１６ 例其他肿瘤中， 表达率依次为：ｇｃｙｓ１０ 为３７－５ ％ ，ｇｃｙｓ１ 为１４－３ ％ ，ｇｃｙｓ１８ 为２７－５ ％ ，ｇｃｙｓ１１为４３－７ ％ ，ｇｃｙｓ４ 为１４－３ ％ ，ｇｃｙｓ８ 为３１－３ ％ ；③３３ 例萎缩性胃炎中，表达率依次为：ｇｃｙｓ１０ 是４０－５ ％ ，ｇｃｙｓ１ 是１６－７ ％ ，ｇｃｙｓ１８ 为４４－５ ％ ，ｇｃｙｓ１１ 为４３－３ ％ ，ｇｃｙｓ４ 为３３－３ ％ ，ｇｃｙｓ８为４８－３ ％ ；２８ 例肠腺化生

Screening and its clinical significance of 6 fragments of highly expressing genes in gastric cancer and precancerous mucosa

AIM To screen several genes which were differentially expressed in gastric cancer and precancerous pathological changes with higher examination rates. METHODS By using gcys 1 to gcys 18 as research targets, 48 specimens of gastric cancers and 16 specimens of other tumors as well as 110 specimens of biospied gastric mucosa were screened by quantitative RT PCR. RESULTS In 48 specimens of gastric cancers, 6 fragments with higher examination rates were gcys 10 (90 5%), gcys 1 (80 9%), gcys 18 (69 5%), gcys 11 (61 9%), gcys 4 (42 85%) and gcys 8 (35 7%). Their positive rates in paratumor tissues were <10%, with a significant difference between gastric cancers and paratumor tissues ( P <0 01). In 16 specimens of other tumors, the expression rates of gcys 10 , 37 5%; gcys 1 , 14 3%; gcys 18 , 27 5%; gcys 11 , 43 8%; gcys 4 , 14 3%; and gcys 8 , 31 2%. In 33 specimens of atrophic gastritis, their expression rates were 40 5%, 16 7%, 44 5%, 43 3%, 33 3%, and 48 3%. In 28 specimens of intestinal metaplasia, their expression rates were 60 5%, 35 3%, 34 5%, 58 8%, 23 8%, and 58 3%. In 17 specimens of atrophic gastritis with intestinal metaplasia, the expression rates were 70 5%, 36 7%, 54 5%, 63 3%, 37 8%, and 68 3%. In 19 specimens of dysplasia, the expression rates were 50 8%, 39 3%, 34 5%, 48 8%, 25 8%, and 48 4%. In 6 gastric ulcers and 7 acute gastritis, the expression rates were 7 5%, 8 0%, 7 5%, 0% and 0%. The expression rates of 6 fragments between precancerous changes and gastric ulcer and gastritis were significantly different ( P <0 01). CONCLUSION gcys 1 , 4, 8, 10, 11, 18 may be correlated with precancerous changes and early gastric cancer. To monitor these 6 genes in gastric mucous may be very important to finding early gastric cancer.