中国苗族人群血管紧张素原和血管紧张素转化酶基因多态性与冠心病的关系

目的 研究血管紧张素原(ACT)基因M235T分子变异和血管紧张素转化酶(ACE)基因I/D多态性与冠状动脉粥样硬化的关系.方法 采用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)技术检测冠心病(CHD)组151例和正常对照组127例AGT基因多态性,采用聚合酶链反应技术检测CHD组151例和正常对照组127例ACE基因I/D多态性.结果 CHD组AGT-TT基因型频率为76.26%,显著高于对照组44.10%(P<0.01).ACE-DD基因型频率为35.10%,显著高于对照组14.96%(P<0.01).结论 在中国苗族人群中,AGT基因TT基因型和AGE基因DD基因型是CHD发病既相互独立又具有协同作用的危险因子.

Association Between the Gene Polymorphism of Angiotensinogen and Angiotensin Coverting Enzyme and Coronary Artery Disease

Aim To investigate the relation between the polymorphisms of angiotensinogen(AGT) genetpye M235T and angiotensin-converting enzyme(ACE) genetpye I/D and coronary atherosclerosis. Methods The study population was consisted of 151 patients with coronary heart disease(CHD),the healthy control group was consisted of 127 without CHD.Patients with CHD were compared with those persons in the healthy control group.AGT gene polymorphisms was tested with polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) technology and ACE gene polymorphisms was tested with PCR. Results The ratio of AGT-TT and ACE-DD genetype in CHD group was separately 76.26% and 35.10%,which was significantly higher than the control group(44.10% and 14.96%).There was significant difference of the AGT-TT genetype between the CHD group and the control group P><0.01;so was ACE-DD genetype between the two group(P><0.01). Conclusion In the population of Chinese miao nationality,the gene polymorphisms of genetype AGT-TT and genetype ACE-DD are two risk factors of CHD morbidity and they are both independent of each other but also mutually coordinated.