氟桂利嗪对青霉素致癎大鼠海马细胞单位放电特征的影响

目的：探讨青霉素癫癎模型大鼠海马神经元单位放电的特征。方法：36只Wistar雄性健康大鼠，随机分为正常对照组、癫癎模型组、癫癎预处理组，各12只。①正常对照组记录单位放电后处死；②癫癎模型组用青霉素钠按600万U／kg体重腹腔注射制作成癫癎模型，记录单位放电后处死；③癫癎预处理组制作模型前用盐酸氟桂利嗪按20mg／kg体重每隔12h灌胃共两次，第二次给药后2h制作成癫癎模型，然后作单位放电记录后处死。结果：①正常对照组大鼠共记录到24个单位海马神经元放电；②癫癎模型组共记录到78个单位海马神经元放电；③癫癎预处理组共记录到47个单位海马神经元放电。与正常对照组比较，癫癎模型组海马神经元单位放电频率增加，放电形式多为混合型，差异有极显著意义（P〈0．01）；与癫癎模型组比较，癫癎预处理组海马神经元单位放电频率降低，差异有统计学意义（P〈0．05）。结论：①癫癎大鼠是以暴发放电为主的混合型放电，抽搐时多表现为束簇状；②盐酸氟桂利嗪对癫癎大鼠放电有显著的抑制作用。

Characteristics of unit discharge in the hippocampal neurons of epileptic rats

Objective： To investigate the characteristics of unit discharge in ;the hippocampat neurons of epileptic rats . Methods： Thirty-six male and healthy Wistar rats were divided into the control group, the epileptic model group and the epileptic pretreatment group. ①Rats of the control group are executed after the unit discharge were recorded; ②In the epileptic model group epileptic model were made using henzylpenicliin sodium as 6000000 unit/kg by intraperiton injection,and the group rats are executed after the unit discharges; ③Before the epileptic pretreatment models were made, flunarizine in the dosages as 20mg/kg were injected into the stomach of rats every 12 hours, up to twice,two hours after the second injection. The epileptic model rats are executed after recording the unit discharge. Results：①Twenty-four unit hippocampal discharges were recorded in the controls. ②78 unit hippocampal discharges were recorded in the epileptic model group. ③47 unit hippocampal discharges were recorded in the epileptic therapy group. Compared with controls, the frequencies of unit hippocampat discharges increase , and many of discharge forms were complex, and the difference has statistical significance （P〈 0.01 ）;Compared with the epileptic model group, the difference of frequencies and forms of unit hippocampal discharges had sta tistical significance（P〈0.05） . Conclusion： ②Complex discharges are observed in epileptic rats,mainly burst discharge. Bunchy or clusty discharges are observed mainly when epilepsy is attacking. ③Calcium antagonist forexample flunarizine may decrease unit discharge and clinical epileptic attack .