慢性肝炎患者乙型肝炎病毒复制与肝纤维化标志物的关系

目的研究慢性乙型肝炎（下称乙肝）患者血清乙肝病毒（HBV）复制水平与肝纤维化血清标志物的关系。方法选择150例临床确诊为慢性乙肝的50例早期肝硬化患者，采用荧光定量聚合酶链反应（FQ-PCR）检测血清HBVDNA水平，放射免疫法和酶免疫法检测肝纤维化血清标志物（透明质酸、层黏连蛋白、Ⅲ型前胶原和Ⅳ型胶原），对血清HBVDNA水平与肝纤维化标志物的关系进行分析，并与100例无肝硬化患者血清HBVDNA及肝纤维化标志物水平进行比较。结果慢性乙肝患者血清HBVDNA水平与肝纤维化标志物的关系无统计学意义（P〉0．05），早期肝硬化患者血清肝纤维化标志物水平显著高于无肝硬化患者，但HBVDNA水平却低于无肝硬化患者（P〈0．05）。结论慢性乙肝患者血清HBVDNA水平与肝纤维化标志物水平无显著相关性。

Relationship between serum HBV DNA levels and hepatic fibrosis markers in chronic hepatitis B

Objective To study the relationship between serum HBV DNA levels and hepatic fibrosis markers in chronic hepatitis B. Methods 150 patients with chronic hepatitis B were included in the study,50 patients among them were diagnosed as early cirrhosis by liver biopsy. Serum HBV DNA levels were determined using fluorescent quantitative PCR, and the hepatic fibrosis markers including hyaluronic acid （HA）, laminin （LN）, aminoterminal propeptide of type Ⅲ precollagen（PⅢP）and type Ⅳ collagen（IV-C）were determined by radioimmunityassay. The relationship between serum HBV DNA levels and hepatic fibrosis markers were analyzed. Serum HBV DNA levels and hepatic fibrosis markers of 50 patients with early cirrhosis were compared with those of 100 non-cirrhotic patients. Results There was no significant relationship between serum HBV DNA levels and hepatic fibrosis markers in chronic hepatitis B（P〉0.05）. The patients with early cirrhosis had much higher hepatic fibrosis markers together with lower HBV DNA levels than non-cirrhotic patients （P〈0.05）. Conclusion There is no significant relationship between serum HBV levels and hepatic fibrosis markers in the patients with chronic hepatitis B DNA.