| HPLC法测定治带颗粒中原儿茶酸的含量 |
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| 引用本文： | 吴声振,雷凯君. HPLC法测定治带颗粒中原儿茶酸的含量[J]. 广东药学院学报, 2011, 27(2): 157-159. DOI: 10.3969/j.issn.1006-8783.2011.02.013 |
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| 作者姓名： | 吴声振 雷凯君 |
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| 作者单位： | 佛山市中医院药剂科,广东,佛山,528000 |
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| 摘 要： | 目的 建立治带颗粒中原儿茶酸的含量测定方法.方法 采用高效液相色谱法,以Hypersil C18(4.6mm×250mm,5μm)为色谱柱;甲醇-水-冰醋酸(体积比19∶80∶1)为流动相,260 nm为波长检测,流速为1.0 mL·min-1的色谱条件进行测定.结果 原儿茶酸在0.0512～0.768 0μg范围内有...
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| 关 键 词： | 治带颗粒 高效液相色谱法 原儿茶酸 |
Determination of protocatechuic acid in Zhidai granules by HPLC |
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| WU Sheng-zhen,LEI Kai-jun. Determination of protocatechuic acid in Zhidai granules by HPLC[J]. Academic Journal of Guangdong College of Pharmacy, 2011, 27(2): 157-159. DOI: 10.3969/j.issn.1006-8783.2011.02.013 |
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| Authors: | WU Sheng-zhen LEI Kai-jun |
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| Affiliation: | (Department of Pharmaceutics,Foshan Hospital of Traditional Chinese Medicine,Foshan,Guangdong 528000,China) |
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| Abstract: | Objective To establish a HPLC method to the assay of protocatechuie acid in Zhidai granules. Methods Protocatechuic acid was determined by Hypersil Cls column （4.6 mm ×250 mm, 5 μm） using methanol-water-acetic acid（ 19：80：1 ） （~）as mobile phase with the flow rate of 1.0 mL ·min^-1 and the detection wavelength was 260 nm. Results There was a good linear relationship between the concentration and peak area value of protocatechuic acid in the range of 0. 051 2 -0. 768 0 μg （r = 0. 999 8）. The average recovery was 100. 90% with the RSD of 1.79% （ n = 6 ）. Conclusion This method is specific, reliable, reproducible and feasible, which can be used for quality control of Zhidai granules. |
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| Keywords: | Zhidai granules HPLC protocatechuic acid |
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