宫颈癌前病变中ΔNP63与HPV16 E7表达的研究

目的:研究宫颈上皮内瘤变中ANP63和HPV16 E7 mRNA的表达情况,寻找用于临床评估CIN病变进展趋势的生物学标志物。方法:收集临床71例薄层液基细胞标本,采用SP免疫组化法检测细胞中ANP63的蛋白表达,RT-PCR法检测残留标本中HPV16 E7的mRNA转录情况。结果:1．ANP63定位于异常鳞状上皮细胞和腺细胞,在CIN中表达高于炎症组(P< 0．05);2．HPV16 E7在CIN中表达明显高于炎症组(P<0．05);3．CIN中ANP63与HPV16 E7的检测结果具有较好的一致性。结论:ANP63可作为CIN的生物学标志物辅助细胞学筛查;ANP63可间接反映HPV16 E7的转录情况,有利于临床评估高危型病毒HPV16的致瘤能力。

Study on Expression of ANP63 and HPV16 E7 in Cervical Intraepithelial Neoplasia

objective: To examine the potential of ANP63 and HPV16 E7 as one of biomarkers for cervical premalignant lesions in Thi-nPrep smears, and to search for the identification of specific biomarkers for cervical intraepithelial neoplasia (CIN) as a new examining method to evaluate evolution of CIN. Method; Streptavidin peroxidase (SP) method was used. Immunocytochemical analysis of ANP63 expression was performed in 71 ThinPrep smears. RT - PCR was used to detect the mRNA of HPV16 E7 in 71 ThinPrep liquid - based smears specimens. Result; Positive expression rate of ANP63 in CIN was higher than that in inflammation (P <0.01). The positive expression rate of HPV16 E7 in CIN was higher than that in inflammation (P < 0.01). Conclusion; Overexpression of ANP63 can be a useful marker to reduce false - negative cytology interpretation. ANP63 should show indirectly the activation of HPV16 E7, which should be beneficial to evaluate the tumorigensis of HPV 16.