基于靶点“钩钓”策略的首荟通便胶囊肠道直接作用靶点鉴定

基于靶点“钩钓”策略鉴定首荟通便胶囊发挥润肠通便作用的直接药理靶点,探索靶点相关药理学信号通路,揭示润肠通便机制.首先制备键合首荟通便胶囊药材总提取物的磁性固相微球,然后从大鼠肠道总蛋白裂解液中捕获直接作用靶点蛋白,并通过高分辨质谱进行鉴定,最后利用Cytoscape软件进行通路富集分析.共计发现138个首荟通便胶囊的...

Identification of intestine direct targets of Shouhui Tongbian Capsules using"target fishing"strategy

"Target fishing"strategy was used to investigate the direct targets and mechanism of Shouhui Tongbian Capsules on relaxing bowel.Magnetic beads cross-linked with the chemical constituents from Shouhui Tongbian Capsules were prepared.The potential target proteins were captured from the total protein lysates of rat intestine using the beads.The captured proteins were further identified by LC-MS/MS,and the associated pathways were analyzed by Cytoscape.Results showed that 138 potential target proteins were identified,which were involved in eight signaling pathways,including tricarboxylic acid cycle,pyrimidine metabolism,sulfur metabolism,fatty acid degradation,alanine/aspartate/glutamate metabolism,arginine/proline metabolism,valine/leucine/isoleucine degradation,andβ-alanine metabolism.Taken together,Shouhui Tongbian Capsules may exert relaxing bowel effect by acting on multiple signaling pathways to promote intestinal gurgling,inhibit inflammation,as well as improve intestinal barrier function,intestinal water secretion,and intestinal flora.