天香丹对ApoE(-/-)基因敲除小鼠动脉粥样硬化的影响

目的:观察天香丹对ApoE(-/-)基因敲除小鼠高脂血症及动脉粥样硬化的影响.方法:将30只8周龄的ApoE(-/-)基因敲除小鼠随机分为模型组、天香丹组和阿托法汀组,每组各10只,另以相同遗传背景的同龄正常C57BL/6J小鼠为正常对照组(n=10).天香丹组每只灌服天香丹7.2g·kg-1·d-1,阿托伐他汀组每只灌服阿托伐他汀10mg·kg-1·d-1,模型组和正常对照组每只灌服0.9％生理盐水0.2ml·10g-1·d-1.连续灌胃12周后眼眶采血测定各组血脂含量,取小鼠主动脉弓部进行形态学观察及图像分析.结果:模型组血清总胆固醇、甘油三酯和低密度脂蛋白水平明显高于正常对照组(P＜0.05),而天香丹组和阿托伐他汀组以上3项指标与模型组相比明显下降(P＜0.05).图像分析测量结果显示模型组与正常对照组比较其斑块总面积明显增加(P＜0.05)；天香丹组动脉硬化病变程度较模型组减轻,其斑块总面积明显减小(P＜0.05).结论:天香丹有降低ApoE(-/-)基因敲除小鼠血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平及减缓动脉粥样硬化病变的作用.

Therapeutic efficacy of Tianxiangdan on atherosclerosis in ApoE(-/-) gene knockout mice

Objective: To study the therapeutic efficacy of Tianxiangdan on hyperlipemia and atherosclerosis in ApoE(-/-) gene knockout mice.Methods: Thirty 8-week ApoE(-/-) gene knockout mice were randomly divided into model group(n=10),Tianxiangdan group(n=10),and atorvastatin group(n=10),and 10 normal C57BL/6J mice with the same genetic background and of the same age were used as normal control group.Each mouse in the Tianxiangdan group was orally given Tianxiangdan at 7.2g·kg-1·d-1.Each mouse in the atorvastatin group was orally given atorvastatin at 10 mg·kg-1·d-1.Each mouse in the model group and normal control group was orally given 0.9% saline at 0.2ml·10g-1·d-1.Blood samples were taken from the eye orbit after the mice were treated for 12 weeks to measure the blood fat level.The aortic arches of the mice were collected for morphological observation and image analysis.Results: The total cholesterol(TC),total triglyceride(TG),and low density lipoprotein(LDL) in the model group were significantly higher than those in the normal control group(P<0.05),while the above indices in the Tianxiangdan group and atorvastatin group were significantly lower than those in the model group(P<0.05).The image analysis showed that the model group had a significantly enlarged total plaque area as compared with the normal control group(P<0.05).The Tianxiangdan group showed reduced atherosclerosis and significantly decreased total plaque area as compared with the model group(P<0.05).Conclusion: Tianxiangdan can decrease TC,TG,and LDL and retard atherosclerosis in ApoE(-/-) gene knockout mice.