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| 应用引物原位标记快速产前诊断20例胎儿染色体非整倍体 | |
| 引用本文： | 舒群,王莉敏,王德芬,王怀军,庄依亮.应用引物原位标记快速产前诊断20例胎儿染色体非整倍体[J].中国实用妇科与产科杂志,2003,19(1):28-30. |
| 作者姓名： | 舒群 王莉敏 王德芬 王怀军 庄依亮 |
| 作者单位： | 1. 上海市第一妇婴保健院,200040 2. 辽阳市第三人民医院 3. 复旦大学附属妇产科医院 |
| 基金项目： | 上海市科委科学基金资助 (资助号:98- 2 - 30 2 ) |
| 摘 要： | 目的：探讨引物原位标记（PRINS）应用于脐血间期细胞进行快速产前诊断染色体非整倍体的准确性及可行性。方法：采用18，21，13，X，Y染色体特异性引物，检测20例脐血间期细胞染色体数目，并与染色体G带分析结果比较。结果：采用PRINS方法检测20例脐血标本，检出1例13－三体，1例18－三体，2例21－三体，与染色体G带分析结果一致，准确率达100％。结论：PRINS产前诊断染色体数目异常，具有准确性高、快速、简便、价廉的特点，值得进一步研究与推广。 |
| 关 键 词： | 引物原位标记 产前诊断 胎儿 染色体 非整倍体 |
| 文章编号： | 1005-2216(2003)01-0028-03 |
Rapid prenatal diagnosis of 20 cases of fetal chromosomal aneuploidies by primed in situ labeling (PRINS) |
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| Shu Qun ,Wang Limin,Wang Defen,et al..Rapid prenatal diagnosis of 20 cases of fetal chromosomal aneuploidies by primed in situ labeling (PRINS)[J].Chinese Journal of Practical Gynecology and Obstetrics,2003,19(1):28-30. | |
| Authors: | Shu Qun Wang Limin Wang Defen |
| Institution: | Shu Qun *,Wang Limin,Wang Defen,et al. *First Maternity & Infant Health Hospital of Shanghai,Shanghai 200040,China |
| Abstract: | Objective To study the feasibility of PRINS for rapid prenatal diagnosis of fetal aneuploidy in interphase cells from umbilical blood.Methods Primer 13?18?21? X and Y were used to label chromosomes in interphase cells from umbilical blood. The results of PRINS were compared with the chromosome G banding analysis.Results Using the primer to label chromosomes in interphase cells from umbilical blood,one sample of trisomy 13,one sample of trisomy 18,two samples of trisomy 21 were detected and all gender of fetus were judged correctly.Conclusion PRINS is a rapid?efficient and reliable method to detect fetal aneuploidy in interphase cells from umbilical blood.We think that it is an efficient way for rapid prenatal diagnosis of aneuploidy and deserved to be popularized. |
| Keywords: | PRINS (Primed in situ labeling) Umbilical blood Prenatal diagnosis Aneuploidy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |