| 单抗检测痰液脱落细胞的诊断学意义 |
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| 引用本文： | 许秀芳,沈毅,王晶,龚非力,阎锡蕴.单抗检测痰液脱落细胞的诊断学意义[J].中德临床肿瘤学杂志,2003,2(2):110-113. |
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| 作者姓名： | 许秀芳 沈毅 王晶 龚非力 阎锡蕴 |
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| 作者单位： | 武汉华中科技大学同济医学院免疫学研究所,武汉华中科技大学同济医学院免疫学研究所,武汉华中科技大学同济医学院免疫学研究所,武汉华中科技大学同济医学院免疫学研究所,武汉华中科技大学同济医学院免疫学研究所 430030 北京心肺血管疾病研究所100029,430030,430030,430030,430030 |
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| 基金项目： | This project was supported by a grant from National Natural Science Founda-
tion of China(No.C03031906). |
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| 摘 要： | 目的 建立一种快速而特异的肺癌辅助诊断方法。方法 制备特异性识别肺癌细胞的鼠单克隆抗体,通过细胞免疫化学和ELJSA方法检测抗体与痰液中脱落细胞的反应情况。结果 两种方法都显示,抗体2C25可选择性结合肺癌患者痰液中的脱落细胞,而不与肺炎患者和正常人痰液中的细胞发生反应。结论 抗体2C25在肺癌痰液免疫诊断中具有潜在的临床应用价值。
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| 关 键 词： | 单克隆抗体 肺癌 诊断 |
| 收稿时间： | 4 April 2003 |
Immuno-detection of exfoliated cells in sputum with a monoclonal antibody |
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| Xu?Xiufang,Shen?Yi,Wang?Jing,Gong?Feili,Yan?XiyunEmail author.Immuno-detection of exfoliated cells in sputum with a monoclonal antibody[J].The Chinese-German Journal of Clinical Oncology,2003,2(2):110-113. |
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| Authors: | Xu?Xiufang Shen?Yi Wang?Jing Gong?Feili Email author" target="_blank">Yan?XiyunEmail author |
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| Institution: | (1) Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China;(2) Present address: Beijing Institute of Heart, Lung and Blood Vessel Disease, 100029 Beijing, China;(3) Biophysics Institute, Chinese Academy of Sciences, 100101 Beijing, China;(4) Molecular Biology Center of Institute of Biophysics, Academy of Science, 100101 Beijing, China |
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| Abstract: | Objective To develop a prompt and specific method for diagnosis of lung cancer.
Methods A murine monoclonal antibody against lung cancer cells was developed and characterized with the techniques of ELISA, immunohistochemistry and immunocytochemical detection of sputum.
Results The antibody selectively bound to lung cancer tissues and exfoliated cells in the sputum from the patients with lung cancer , but did not bind to normal lung tissues and the cells in sputum either from the patients with pneumonia or from normal individuals. Conclusion The antibody 2C2S has potential application for immunocytological detection of lung cancer cells in sputum. |
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| Keywords: | monoclonal antibody lung cancer diagnosis |
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