雌激素与抗雌激素制剂对子宫内膜癌细胞株化疗效应的研究

目的 探讨雌激素与抗雌激素制剂对子宫内膜癌细胞化疗效应的影响。方法 采用细胞凋亡ELISA方法检测雌激素、三苯氧胺与阿霉素相互作用后,ER( )RL-952子宫内膜癌细胞的凋亡水平。结果 阿霉素诱发：ER( )RL-952子宫内膜癌细胞产生一定的细胞凋亡水平,雌激素能阻断阿霉素的作用,使细胞凋亡水平下降,呈剂量依赖反应;三苯氧胺也能诱发细胞凋亡,与阿霉素作用后,促进子宫内膜癌细胞凋亡,高于三苯氧胺单一作用产生的细胞凋亡水平,并与三苯氧胺浓度剂量呈正相关。结论 雌激素能阻断阿霉素对子宫内膜癌细胞的细胞凋亡作用,而三苯氧胺能促进阿霉素的作用,三苯氧胺与阿霉素的联合应用有助于为子宫内膜癌临床治疗的选择提供依据。

Effect of estrogen and antiestrogen on chemotherapeutic sensitivity of endometrial carcinoma cell line

Objective: To study the effect of estrogen and tamoxifen on chemotherapeutic sensitivity in ER(+) endometrial carcinoma cells.Methods: DNA fragmentation as the criteria for apoptotic cell death was used to evaluate the value of estrogen, tamoxifen and adriamycin in ER(+) endometrial carcinoma cells. DNA fragmentation was measured with the cell death ELISA.Results: Adriamycin and tamoxifen could induce apoptosis in ER(+) endometrial carcinoma cell. The cell apoptosis level was decreased with the increasing of 17-β-estradiol concentration (P<0.001) and was inversely proportional to 17-β-estradiol concentration (IgM) (P<0.01). The cell apoptosis level was increased with the increasing of tamoxifen concentration (P<0.01) and was also directly proportional to tamoxifen concentration (IgM). Furthermore, the cell apoptosis level was increased significantly after treated with both tamoxifen and adriamycin.Conclusion: Estrogen may block apoptosis induced by adriamycin in ER(+) endometrial carcinoma cell. Tamoxifen can increase the sensitivity of endometrial carcinoma cell to adriamycin. Tamoxifen combined with chemotherapeutic drug may be of significant therapeutic benefit in ER(+) endometrial carcinoma.