扶正抑瘤颗粒对小鼠H22肿瘤细胞凋亡及p53和Caspase-3基因表达的影响

目的 研究扶正抑瘤颗粒(FYK)对小鼠H22肿瘤细胞生长的抑制作用，及其对细胞凋亡和p53、Caspase-3基因表达的影响。方法 用体内实验观察FYK对H22瘤株的抑瘤率，流式细胞术测定细胞凋亡率和细胞周期，反转录-聚合酶链式反应(RT-PCR)法测定野生型p53(Wtp53)和Caspase-3的mRNA表达情况。结果 12g／kg、24g／kg FYK均能显著抑制小鼠H22肿瘤细胞的生长，最高抑瘤率为51．24％(P<0．01)。流式细胞术检测表明，FYK显著提高H22肿瘤细胞的凋亡率，最高为15．84％(P<0．01)；肿瘤细胞阻滞在G0／G1期，S期细胞比率降低；RT-PCR实验表明，FYK可显著上调p53和Caspase-3 mRNA的表达水平。结论 FYK能显著抑制小鼠H22肿瘤细胞生长，其抗肿瘤机制与诱导细胞凋亡，调控细胞周期，促进Wt p53与Caspase-3基因的表达有关。

Effect of Fuzheng Yiliu Granule on H22 Tumor Cell Apoptosis, p53 and Caspase-3 Gene Expression in Mice

ObjectiveTo explore the inhibitory effect of Fuzheng Yiliu Granule (FYG) on growth and apoptosis of H_ 22 tumor cell, and expressions of p53 and Caspase-3 gene. MethodsThe tumor inhibitory rate of FYG on H_ 22 tumor cell line was observed in vivo, cell apoptosis rate and cell cycle were determined by flow cytometry (FCM), and expressions of wild type p53 and Capase-3 mRNA were determined by RT-PCR. ResultsFYG could inhibit the growth of H_ 22 tumor cell at the dose of 12g/kg, 24g/kg, the maximal inhibitory rate up to 51.24% (P<0.01). By FCM, it was shown that FYG could significantly enhance apoptosis rate of cell line H_ 22 , with the peak reached 15.84% (P<0.01),and cause the tumor cell cycle being blocked at G_0/G_1 phase, with decrease of cells in S phase. RT-PCR illustrated that FYG could significantly up-regulate the level of p53 and Caspase-3 mRNA expression. ConclusionFYG can significantly inhibit the growth of tumor cell in mice, its anti-tumor mechanisms may relate to the cell apoptosis induction, cell cycle regulation and wild type p53 and capase-3 gene expression enhancing.