铜绿假单胞菌消毒剂-磺胺耐药基因检测及对消毒剂的抗性研究

目的了解铜绿假单胞菌Ⅰ类整合子消毒剂-磺胺耐药基因(qacE△1-sull)的携带情况以及阳性菌株对碘伏、戊二醛、氯己定、"84"消毒液的抗性。方法对某院2007年12月-2008年1月连续分离的20株铜绿假单胞菌以聚合酶链反应(PCR)法检测qacE△l-sull基因,测定其阳性菌株对上述4种消毒剂的最低抑菌浓度(MIC)。结果 20株铜绿假单胞菌中检出qacE△1-sull阳性株10株(50.00%)。碘伏、戊二醛、氯己定、"84"消毒液对qacE△1-sull阳性菌株的MIC范围分别为:8.0～128.0μg/mL、16.0～256.0μg/mL、1.0～16.0μg/mL、4.0～64.0μg/mL。结论 qacE△1-sul1基因普遍存在于临床分离的铜绿假单胞菌中,阳性菌株对碘伏、戊二醛、氯己定、"84"消毒液存在抗性差异,应合理使用消毒剂并增加作用时间,以有效控制铜绿假单胞菌的播散。

Detection of disinfectant sulfadiazine resistance gene qacEΔ1 sul1 and resistance to Pseudomonas aeruginosa

Objective To explore the distribution of disinfectant-sulfadiazine-resistance gene qacE△1-sul1 in Pseudornonas aeruginosa （P. aeruginosa） and the resistance of positive strains to four kinds of disinfectants （iodophor, glutaraldehyde, chlorhexidine and ＂84＂ disinfectant ）. Methods Twenty strains of P. aeruginosa isolated from a hosptial between December, 2007 and January 2008 were detected qacE△1 sul1 gene by polymerase chain reaction, minimum inhibitory concentrations （MICs） of the above four disinfectants were detected. Results In 20 strains of P. aeruginosa, 10（50. 00%） strains were positive for qacE△1-su1 1 gene. MICs of iodophor, glutaraldehyde, chlorhexidine and ＂84＂ disinfectant to positive qacE△1-sul 1 strains were 8.0 - 128. 0 μg/mL, 16. 0 -256. 0 μg/mL,1.0-16. 0 μg/mL, and 4. 0-64. 0 μg/mL, respectively. Conclusion qacE△1-sul1 gene is widespread in the clinical isolated P. aeruginosa, there are differences in resistance of positive strains against four kinds of disin- fectants, disinfectants should be used rationally so as to control the spread of P. aeruginosa.