| 癌胚抗原检测方法学评价及其在胃癌诊断中的意义 |
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| 引用本文： | 王恩兰,李涛.癌胚抗原检测方法学评价及其在胃癌诊断中的意义[J].标记免疫分析与临床,2005,12(4):235-236,234. |
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| 作者姓名： | 王恩兰 李涛 |
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| 作者单位： | 安徽医科大学第一附属医院检验科,安徽,合肥,230022 |
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| 摘 要： | 为比较临床常用CEA检测方法的特异性和敏感性,探讨CEA在胃癌诊断中的意义,用ELISA、时间分辨荧光免疫法(TRFIA)、RIA、化学发光免疫法(CLIA)检测36例病理确诊胃癌患者血清CEA水平,同时检测20名健康体检者血清CEA水平.结果显示,四种方法的特异性均为100%,ELISA敏感性最低,为19.4%,CLIA的敏感性最高,为44.4%.结论是ELISA检测CEA的敏感性有待提高,CEA检测不能作为胃癌诊断的指标,可以作为部分胃癌患者疗效观察的指标.
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| 关 键 词： | 癌胚抗原 胃癌 酶联免疫吸附法 放射免疫法 时间分辨荧光免疫法 化学发光免疫法 |
| 文章编号： | 1006-1703（2005）04-0235-02 |
| 收稿时间： | 2005-04-14 |
| 修稿时间： | 2005-04-142005-07-11 |
Evaluation of Detection Methodology for Carcinoembryonic Antigen and Application of CEA in Diagnosis of Gastric Cancer |
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| WANG En-lan,LI Tao.Evaluation of Detection Methodology for Carcinoembryonic Antigen and Application of CEA in Diagnosis of Gastric Cancer[J].Labeled Immunoassays and Clinical Medicine,2005,12(4):235-236,234. |
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| Authors: | WANG En-lan LI Tao |
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| Institution: | Clinical Laboratory , The First Affiliated Hospital of Anhui Medical University , Hefei 230022, China |
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| Abstract: | To compare the specificity and sensitivity of different methods in detection of CEA, and to investigate the application of CEA detection in diagnosis of gastric cancer, CEA in serum of 36 patients with gastric cancer and 20 negtive reference serum was detected by ELISA, TR-FIA, RIA and CLIA. The results showed that the specificity of these 4 methods was all 100 % and the sensitivity of ELISA was the lowest (19. 4%) while CLIA was the highest (44. 4%). Therefore, the sensitivity of ELISA should be raised and CEA, besides used as an observation index for curative effects in a part of gastric cancer patients, can not be used in diagnosis of gastric cancer. |
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| Keywords: | Carcinoembryonic antigen Gastric cancer ELISA TRFIA CLIA RIA |
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