| 反义RNA靶序列对大肠杆菌ftsZ基因沉默效果的影响 |
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| 引用本文： | 陈银,钱秀萍,何建勇,戈梅,殷瑜. 反义RNA靶序列对大肠杆菌ftsZ基因沉默效果的影响[J]. 沈阳药科大学学报, 2011, 28(7): 564-568,584 |
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| 作者姓名： | 陈银 钱秀萍 何建勇 戈梅 殷瑜 |
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| 作者单位： | 沈阳药科大学生命科学与生物制药学院;上海交通大学药学院;上海来益生物药物研究开发中心 |
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| 基金项目： | 国家科技重大专项资助项目(2009zx09302-004) |
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| 摘 要： | 目的筛选出能够有效引起大肠杆菌ftsZ基因沉默的反义RNA片段。方法利用携带paired termini结构的质粒模仿天然asRNA的二级结构表达稳定的反义RNA,通过平板生长表象观察、菌体形态变化及液体培养生长速率变化考察了不同反义靶序列对大肠杆菌ftsZ基因沉默效果的影响。结果 ftsZ1、ftsZ4和ftsZ5 3个反义RNA片段可有效引起相关基因沉默。结论获得的ftsZ1、ftsZ4和ftsZ5 3个反义RNA片段可进一步用于ftsZ特异性酶抑制剂的超敏全细胞模型构建。
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| 关 键 词： | ftsZ 反义RNA 基因沉默 大肠杆菌 |
| 收稿时间： | 2010-03-05 |
Effect of different antisense RNA sequence on ftsZ gene silencing in Escherichia coli |
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| CHEN Yin,QIAN Xiu-ping,HE Jian-yong,GE Mei,YIN Yu. Effect of different antisense RNA sequence on ftsZ gene silencing in Escherichia coli[J]. Journal of Shenyang Pharmaceutical University, 2011, 28(7): 564-568,584 |
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| Authors: | CHEN Yin QIAN Xiu-ping HE Jian-yong GE Mei YIN Yu |
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| Affiliation: | 1.School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shanghai Health Creation Center for Biopharmaceutical R & D,Shanghai 200240,China;3.Shanghai Jiao Tong University,Shanghai 200240,China) |
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| Abstract: | Objective To select the right antisense fragments with gene scilencing effects targeting ftsZ in Escherichia coli.Methods A vector with paired termini was to constantly express different asRNAs of ftsZ in Escherichia coli and the gene scilencing effects were observed by morphorlogy observation on plate,microscope and liquid culture.Results Three fragments ftsZ1,ftsZ4 and ftsZ5 were found inhibiting cell division effectively.Conclusions These cells with asRNAs can be further used as novel screenig models for selective ftsZ inhibitors. |
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| Keywords: | ftsZ antisense RNA gene silencing Escherichia coli |
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