| 软组织平滑肌肉瘤染色体3p位点特异性的杂合性缺失 |
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| 引用本文： | Peng H,Yang GH,Bu H,Gou LX. 软组织平滑肌肉瘤染色体3p位点特异性的杂合性缺失[J]. 中华病理学杂志, 2003, 32(2): 124-127 |
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| 作者姓名： | Peng H Yang GH Bu H Gou LX |
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| 作者单位： | 1. 重庆医科大学病理学教研室
2. 610041,成都,四川大学华西医院病理科 |
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| 基金项目： | 纽约中华医学基金会 (CMB)基金资助项目 (0 0 72 2 ) |
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| 摘 要： | 目的 对软组织平滑肌肉瘤(LMS)染色体3p上的5个特异性位点进行微卫星多态性分析,以明确这些位点染色体等位基因杂合性缺失(LOH)情况。方法 采用微卫星DNA-聚合酶链反应—银染法分析22例LMS 3p14．2-pter范围内的5个特异性位点LOH状态。结果 45．4％LMS至少在一个位点出现LOH,以D3s1295最高(36．8％),其次为D3s1289(10．5％),而D3s1293末检测到LOH。不同组织学分级、大小及不同部位的LMS其LOH发生差异无显著性。结论 LMS3p14．2—23范围发生杂合性缺失率较高,D3s1295及D3s1289位点所在的染色体区域及附近可能隐含与平滑肌肉瘤有关的抑癌基因。
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| 关 键 词： | 软组织平滑肌肉瘤 染色体3p位点 特异性 杂合性缺失 |
| 修稿时间： | 2002-08-02 |
Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma |
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| Peng Hui,Yang Guang-hua,Bu Hong,Gou Li-xin. Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma[J]. Chinese Journal of Pathology, 2003, 32(2): 124-127 |
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| Authors: | Peng Hui Yang Guang-hua Bu Hong Gou Li-xin |
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| Affiliation: | Department of Pathology, Huaxi Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To analyze the loss of heterozygosity (LOH) at 5 loci on chromosome 3p in soft tissue leiomyosarcoma (LMS) Methods LOH was detected in 22 cases of LMS using PCR silver staining targeting 5 microsatellite sites on 3p14 2 pter Relation between LOH and LMS clinical pathological features was also analyzed. Results Ten of 22 LMS samples showed LOH at more than one locus (45 4%) Among the 5 loci, LOH occurred more frequently at D3s1295 (36 8%) and D3s1289 (10 5%), but absent at D3s1293 No significant difference was found on LOH incidence between different grade, size and location of LMS Conclusions LOH on chromosome 3p14 2 23 region is relatively frequent in LMS Region around D3s1295 and D3s1289 may harbor tumor suppressor gene relating to LMS |
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| Keywords: | Soft tissue neoplasms Leiomyosarcoma Chromosomes human pair 3 Loss of heterozygosity |
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