| 正交试验优选龙胆中总环烯醚萜苷的提取工艺研究 |
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| 引用本文： | 郭小存,朱鹤云,冯波,周海波,李兵,王帅,臧君家,王海杰. 正交试验优选龙胆中总环烯醚萜苷的提取工艺研究[J]. 吉林医药学院学报, 2011, 32(5): 283-286 |
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| 作者姓名： | 郭小存 朱鹤云 冯波 周海波 李兵 王帅 臧君家 王海杰 |
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| 作者单位： | 吉林医药学院药学院,吉林 吉林,132013 |
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| 基金项目： | 吉林医药学院大学生科研基金资助项目(吉医学科字[2010]第6号) |
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| 摘 要： | 目的优选龙胆中总环烯醚萜苷的提取工艺。方法以总环烯醚萜苷的含量为指标,采用紫外分光光度法进行含量测定。对于冷浸法、超声提取法、回流提取法和索氏提取法提取龙胆中总环烯醚萜苷的工艺进行了比较,并采用L9（34）正交试验设计确定最佳提取工艺。结果回流提取法提取效果最佳,最终优化的提取工艺为：提取溶剂为70%乙醇,提取时间为2.0 h,料液比为1∶15。在此条件下,龙胆总环烯醚萜苷的含量为7.15%。结论该提取工艺具有效率高、时间短、能耗低、环保等优点,可为龙胆总环烯醚萜苷的工业化生产和新药开发提供依据。
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| 关 键 词： | 龙胆 总环烯醚萜苷 紫外分光光度法 正交试验设计 |
Optimization on extraction technology of total iridoid glycoside from Gentiana scabra Bge. by orthogonal design |
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| GUO Xiao-cun,ZHU He-yun,FENG Bo,ZHOU Hai-bo,LI Bing,WANG Shuai,ZANG Jun-jia,WANG Hai-jie. Optimization on extraction technology of total iridoid glycoside from Gentiana scabra Bge. by orthogonal design[J]. Journal of Jilin Medical College, 2011, 32(5): 283-286 |
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| Authors: | GUO Xiao-cun ZHU He-yun FENG Bo ZHOU Hai-bo LI Bing WANG Shuai ZANG Jun-jia WANG Hai-jie |
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| Affiliation: | GUO Xiao-cun,ZHU He-yun,FENG Bo,ZHOU Hai-bo,LI Bing,WANG Shuai,ZANG Jun-jia,WANG Hai-jie(College of Pharmacy,Jilin Medical College,Jilin City,Jilin Province,132013,China) |
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| Abstract: | Objective To optimize the extraction technology of total iridoid glycoside from Gentiana scabra Bge.Methods The extraction technology was optimized by L9(34) orthogonal experimental design with the content of total iridoid glycoside as the index which was determined by ultraviolet spectrophotometric method.The merceration extraction,reflux extraction,ultrasonic extraction and soxhlet extraction process were also compared.Results The optimal extraction technology was reflux extraction with extraction solvent... |
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| Keywords: | Radix Gentianae. total iridoid glycoside ultraviolet spectrophotometric method orthogonal design |
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