| 常用抗肿瘤药物对KBV200耐药细胞株增殖抑制作用及苦参碱的耐药逆转研究 |
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| 引用本文： | 陈鸿雁,王驰,余晓燕,沈娜,张潜英,蒋纪恺. 常用抗肿瘤药物对KBV200耐药细胞株增殖抑制作用及苦参碱的耐药逆转研究[J]. 第三军医大学学报, 2006, 28(11): 1231-1233 |
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| 作者姓名： | 陈鸿雁 王驰 余晓燕 沈娜 张潜英 蒋纪恺 |
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| 作者单位： | 重庆医科大学,附属第一医院耳鼻咽喉科,重庆,400016;重庆医科大学,基础医学院化学教研室,重庆,400016;汕头大学医学院分子生物学实验室,汕头,515031 |
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| 摘 要： | 目的探讨5种常用抗肿瘤药长春新碱、阿霉素、平阳霉素、顺铂、环磷酰胺对KBV200耐药细胞株的增殖抑制作用及苦参碱的耐药逆转作用.方法以喉癌KBV200耐药细胞株为研究对象,采用MTT法观察5种抗肿瘤药物对其增殖抑制作用;并采用MTT法观察苦参碱对KBV200耐药细胞株的耐药逆转作用.结果长春新碱、平阳霉素、环磷酰胺对KBV200耐药细胞株的增殖抑制作用差,而阿霉素和顺铂对KBV200耐药细胞株的抑制作用好;当苦参碱浓度大于200 μg/ml时对KBV200耐药细胞株具有直接抑制作用;苦参碱逆转KBV200耐药细胞株对长春新碱、阿霉素耐药的作用较强,逆转KBV200耐药细胞株对平阳霉素、顺铂、环磷酰胺的耐药作用较弱.结论 KBV200耐药细胞株对长春新碱、平阳霉素、环磷酰胺3种药物有不同程度耐药;苦参碱能逆转KBV200耐药细胞株对长春新碱、阿霉素的耐药.
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| 关 键 词： | 抗肿瘤药物 KBV200细胞株 耐药逆转 苦参碱 |
| 文章编号： | 1000-5404（2006）11-1231-03 |
| 收稿时间： | 2006-03-08 |
| 修稿时间： | 2006-03-082006-03-29 |
Inhibitory effect of 5 chemotherapeutic agents on KBV200 cell line and reverse effect on multidrug resistance of Matrine |
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| CHEN Hong-yan,WANG Chi,YU Xiao-yan,SHEN Na,ZHANG Qian-ying,JIANG Ji-kai. Inhibitory effect of 5 chemotherapeutic agents on KBV200 cell line and reverse effect on multidrug resistance of Matrine[J]. Acta Academiae Medicinae Militaris Tertiae, 2006, 28(11): 1231-1233 |
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| Authors: | CHEN Hong-yan WANG Chi YU Xiao-yan SHEN Na ZHANG Qian-ying JIANG Ji-kai |
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| Affiliation: | 1 Department of Otolaryngology, The First Affiliated Hospital, 2The Preclinical Medicine College, Chongqing University of Medical Sciences, Chongqing 400016, 3Shantou University Medical College, Shantou 515031, China |
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| Abstract: | Objective To observe the inhibitory effect of 5 chemotherapeutic agents including vincristine, adriamycin, pingyangmycin, cisplatin, cyclophosphamide on the KBV200 cell line and to study the reverse effect on the muhidrug resistance of Matrine. Methocls MTT assay was used to investigate the inhibition ability of 5 drugs on the cell line and the reverse effect on the muhidrug resistance of Matrine. Results Vincristine, pingyangmycin, cyclophosphamide had less inhibitory effects than adriamycin, cisplatin on the KBV200 cell line. Matrine had the ability to reverse the muhidrug resistance of the cell line to vincristine, adriamycin. Conclusion KBV200 cell line is multidrug-resistant to vincristine, pingyangmycin, cyclophosphamide. Matrine can enhance the sensitivity of KBV200 to the vincristine, |
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| Keywords: | adriamycin.chemotherapeutic agent KBV200 multidrug resistance cell line reversion of multidrug resistance Matrine |
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