| 吲哚美辛对A549/DDP细胞摄取99Tcm-MIBI的影响及其逆转耐药性的机制 |
| |
| 引用本文： | 才久波,张彩霞,高继东. 吲哚美辛对A549/DDP细胞摄取99Tcm-MIBI的影响及其逆转耐药性的机制[J]. 陕西肿瘤医学, 2010, 18(6): 1072-1074 |
| |
| 作者姓名： | 才久波 张彩霞 高继东 |
| |
| 作者单位： | 中国医科大学附属盛京医院核医学科,辽宁沈阳110004 |
| |
| 基金项目： | 辽宁省重点实验室基金资助项目（编号：20060919） |
| |
| 摘 要： | 目的：探讨吲哚美辛对A549/DDP摄取99Tcm的影响及其逆转耐药性的机制.方法：常规方法培养A549/DDP细胞,首先行99Tcm-MIBI摄取实验,然后用DDP和吲哚美辛干预A549/DDP细胞30min后计算99Tcm-MIBI的摄取率,再分别用RT-PCR,Westen-blot检测mdrl基因,Pg-P蛋白的表达.结果：A549/DDP细胞摄取率在90min时达到高峰（0.83%）,吲哚美辛干预后99Tcm-MIBI摄取比干预前增加,达到3.79%.RT-PCR,Westen-blot结果显示吲哚美辛抑制了mdrl基因和Pg-P蛋白的表达.结论：吲哚美辛能提高A549/DDP细胞99Tcm摄取率并通过抑制mdrl和Pg-P的表达逆转肺癌耐药.99Tcm放射性摄取率可作为评价肿瘤耐药的一种方法.
|
| 关 键 词： | 吲哚美辛 肺癌 99Tcm-MIBI mdr |
Effect of Indomethacin on ^99Tcm-MIBI uptake in cisplatin-resistant human lung cancer cells A549/DDP and its mechanism |
| |
| Affiliation: | CAI Jiu - bo, ZHANG Cai - xia, GAO Ji - dong Department of Nuclear Medicine, China Medical University Affiliated Shengiing Hospital, Shenyang 110004, China. |
| |
| Abstract: | Objective： To explore the mechanism of indomethacin reversal cisplatin resistance on lung cancer cells. Methods： With the conventional method,to cuhivat A549 / DDP cells. To do99Tcm -MIBI intake experiment first, then use DDP and indomethacin intervent cell . After 30mins to calculate cells 99Tern - MIBI uptake, then with RT- PCR,western- blot to detect- MDR1 gene and Pg -p protein expression. Results：A549/DDP cells, uptake of 99Tern -MIBI aehived peak in 90 rain （0. 83% ）, 99 Tom -- MIBI intake of indomethacin intervention group increased than DDP intervention group significantly （3.97%） ,Rusults of RT- PCR ,and Western- blot showed indomethacin coued inhibit MDR1 genes and Pg -p protein expression. Conclution： Indomethacin can inhibit MDR1 ,Pg - p ex- pression and reversedrag drag resistance of lung cancer. |
| |
| Keywords: | indomethacin lung cancer ^99Tcm-MIBI mdr |
| 本文献已被 维普 等数据库收录！ |
|
