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| 鲁米诺-过氧化氢化学发光法动态监测硝普钠在小鼠体内释放NO过程 | |
| 引用本文： | 周李承,章林慧,梁桂媛,周宜开,郝巧玲.鲁米诺-过氧化氢化学发光法动态监测硝普钠在小鼠体内释放NO过程[J].华中科技大学学报(医学版),2003,32(4):378-380. |
| 作者姓名： | 周李承 章林慧 梁桂媛 周宜开 郝巧玲 |
| 作者单位： | 1. 华中科技大学同济医学院公共卫生学院环境医学研究所,武汉,430030 2. 欧意药业集团公司,石家庄,050051 3. 华中科技大学同济医学院药学院无机化学教研室,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目 (No .39990 5 70 ) |
| 摘 要： | 目的建立一套快速有效的测量NO的方法并用来监测降压药硝普钠在小鼠体内释放NO的过程。方法建立并采用鲁米诺一过氧化氢(Luminol-H2O2)化学发光体系，动态监测小鼠静脉注射硝普钠(2mg／kg)后血样中NO浓度的变化。结果Luminol-H2O2化学发光体系构建成功，以此测量NO标准系列线性良好；硝普钠给药后，小鼠体内NO30s左右达到峰值，200s恢复给药前水平。结论Luminol-H2O2化学发光体系测量NO灵敏度高、特异性好；硝普钠在体内快速释放NO，硝普钠舒张血管降低血压的作用是通过释放NO实现的。 |
| 关 键 词： | 一氧化氮 硝普钠 化学发光分析 药理 小鼠 |
Dynamic Monitoring of Nitric Oxide in vivo Release Procedure in Rats Following Administration of Sodium Nitroprusside by Luminol-H2O2 Chemiluminescence System |
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| Zhou Licheng ,Zhang Linhui ,Liang Guiyuan et al Institute of Environmental Medicine,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Ouyi Medical Group Company,Shijiazhuang.Dynamic Monitoring of Nitric Oxide in vivo Release Procedure in Rats Following Administration of Sodium Nitroprusside by Luminol-H2O2 Chemiluminescence System[J].Journal of Huazhong University of Science and Technology(Health Sciences),2003,32(4):378-380. | |
| Authors: | Zhou Licheng Zhang Linhui Liang Guiyuan Institute of Environmental Medicine School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan Ouyi Medical Group Company Shijiazhuang |
| Institution: | Zhou Licheng 1,Zhang Linhui 2,Liang Guiyuan 3 et al 1 Institute of Environmental Medicine,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2 Ouyi Medical Group Company,Shijiazhuang 050051 3 Department of Inorganic Chemistry,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
| Abstract: | Objective To establish an effective system to detect the nitric oxide release procedure in the rats after intravenous administration of sodium nitroprusside. Methods A luminol H 2O 2 chemiluminescence system was established to continuously detect the changes of blood nitric oxide (NO) in the rats after intravenous injection of sodium nitroprusside (2 mg/kg). Results The luminol H 2O 2 chemiluminescence system was established successfully with a good linearity to NO standard series. After intravenous injection of sodium nitroprusside, the NO in the rats reached its peak at 30 s and returned to the level at 200 s before administration. Conclusion The luminol H 2O 2 chemiluminescence system has a high sensitivity and good specificity to NO, and the results indicate that sodium nitroprusside takes effects by releasing NO in the body. |
| Keywords: | nitric oxide sodium nitroprusside chemiluminescence analysis |
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