| 人重组pcDNA3人钠/碘同向转运体核表达质粒的构建 |
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| 引用本文： | 张宏飞,严煜,张裕东.人重组pcDNA3人钠/碘同向转运体核表达质粒的构建[J].南通医学院学报,2006,26(4):242-243,246. |
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| 作者姓名： | 张宏飞 严煜 张裕东 |
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| 作者单位： | 南通大学附属医院心胸外科,南通,226001 |
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| 摘 要： | 目的:获取、构建并鉴定携带人钠/碘同向转运体(hNIS)基因的pcDNA3.0质粒载体。方法:利用逆转录和聚合酶链反应获取Graves甲亢患者甲状腺NIS基因可编码区片段。与pUCm-T质粒载体连接、经转化、蓝白斑筛选、酶切鉴定,序列测定鉴定,获得pUCm-T-重组质粒。pUCm-T-重组质粒亚克隆得到pcDNA3-hNIS重组质粒。结果:所构建的pcDNA3-hNIS重组质粒中的hNIS基因序列与文献报道序列一致。结论:成功构建pcDNA3-hNIS重组质粒。
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| 关 键 词： | 钠/碘同向转运体 基因转移 基因治疗 逆转录聚合酶链反应 |
| 文章编号： | 1000-2057（2006）04-0242-03 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
Construction of recombinant human eukaryotic expression vector pcDNA3-hNIS |
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| ZHANG Hongfei,YAN Yu,ZHANG Yudong.Construction of recombinant human eukaryotic expression vector pcDNA3-hNIS[J].ACTA Academiae Medicinae Nantong,2006,26(4):242-243,246. |
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| Authors: | ZHANG Hongfei YAN Yu ZHANG Yudong |
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| Institution: | Department of Cardio - thoracic Surgery, Affiliated Hospital of Nantong University, Nantong 226001 |
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| Abstract: | Objective:To construct and identify recombinant human eukaryotic expression vector with the Sodium/ Iodide symporter (NIS ) genes. Methods:Human Sodium/ Iodide symporter genes cDNA were amplified from thyroid tissue of the patient suffering from Graves disease by Rt - PCR , and cloned into pUCm-T-hNIS , blue/ white color selection and restriction enzyme analysis. pUCm-T-hNIS was sequenced and subcloned into pcDNA3-hNIS. Results:The results showed that the sequence data of hNIS gene in recombinant plasmid corresponded to those of reference report. Conclusion:Successfully constructed pcDNA3-hNIS recombinant plasmid can provide academic evidence for further research. |
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| Keywords: | Sodium/ Iodide symporter Gene transformation Gene therapy Reverse trancriptase polymerase chain reaction |
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