酶联免疫吸附试验检测炭疽病人血清抗毒性抗体的研究

用ELISA检测17例临床确诊的皮肤炭疽病例,其发病后不同时期49份血清中的炭疽抗毒性抗体阳性率达80～100％。炭疽接触者20份血清的阳性率为50～60％。健康人20份血清抗体水平较低。各组间抗毒性抗体的光密度值比较,相差非常显著。以荚膜荧光抗体染色法检测上述血清中荚膜抗体,健康人和炭疽接触者均呈阴性,而炭疽病人阳性率为93.8％。同一病例发病后一个月,抗毒性抗体水平最高,随发病时间延长,抗体水平逐渐下降,但个别病例在一年以后抗毒性抗体仍可维持较高水平。炭疽病人血清中抗毒性抗体与荚膜抗体同时存在,两者之间未见平行关系。

ELISA for detecting antibodies to anthracic toxin in sera from patients.

The antibodies to Anthracic toxin was detected by ELISA in 49 sera from 17 patients with diagnosed cutaneous Anthrax, at different stages.The positivity rate was 80-100%.Of 20 sera from those who had contacted with the patients, the positivity rate was 50-60%.There was a lower level of antibodies to anthra-cic toxin in 20 sera from healthy persons.As compared with antibodies between the groups, the O.D varied greatly.Both of the sera from the healthy persons and the contact with anthrax had been detected, and antibodies against anthrax capsule were negative by the immunofluorescent technique.Its positivity rate in patients was 93.8%. In a month after suffering from anthrax, there was the highest level of anti-toxic Ab in the same patient and the level of Ab was loweras time went on, but the Ab level varied greatly from patient to patient. The le vel of Ab in some patients was still higher than others in one year; both of the anti-toxic Ab and anti-capsule Ab in sera from patients with anthrax were found to exist in serum at the same time, but the correlationship between them ha ven't been found up to now.