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| 正交试验优选马齿苋提取物制备工艺研究 | |
| 引用本文： | 侯银环,辛海量,徐燕丰,岳小强,李敏,路金才,凌昌全.正交试验优选马齿苋提取物制备工艺研究[J].药学服务与研究,2008,8(3):201-203. |
| 作者姓名： | 侯银环 辛海量 徐燕丰 岳小强 李敏 路金才 凌昌全 |
| 作者单位： | 1. 沈阳药科大学中药学院生药学教研室,沈阳,110016 2. 第二军医大学长海医院中医科,上海,200433;中国人民解放军第546医院中医科,新疆马兰,841700 3. 第二军医大学长海医院中医科,上海,200433 4. 第二军医大学海医系军队卫生教研室,上海,200433 |
| 基金项目： | 总后专项项目 , 军队中医药研发推广专项课题 |
| 摘 要： | 目的：优选马齿苋提取物的制备工艺条件。方法：采用L9（3^4）正交试验设计，以药材中α-亚麻酸转移率、浸膏得率和浸膏中α-亚麻酸的含量作为考察指标，采用HPLC法测定α-亚麻酸含量，并对所得结果进行方差分析，综合3个指标结果确定最佳工艺。结果：优选出的最佳工艺条件为将马齿苋提取液回收至无乙醇，加水至0．5g／mL，用10％的NaOH溶液调pH为7．5～8．0，5000r／min离心1min，沉淀加水至1g／mL，超声10min，5000r／min离心1min。结论：优选出的制备工艺稳定可靠，简便可行。 |
| 关 键 词： | 马齿苋 α-亚麻酸 正交试验 工艺学 制药 |
Study on the optimization of preparation technology of extract of Portulaca oleracea L. with orthogonal test |
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| HOU Yin-huan,XIN Hai-liang,XU Yan-feng,YUE Xiao-qiang,LI Min,LU Jin-cai,LING Chang-quan.Study on the optimization of preparation technology of extract of Portulaca oleracea L. with orthogonal test[J].Pharmaceutical Care and Research,2008,8(3):201-203. | |
| Authors: | HOU Yin-huan XIN Hai-liang XU Yan-feng YUE Xiao-qiang LI Min LU Jin-cai LING Chang-quan |
| Affiliation: | HOU Yin-huan ,XIN Hai-liang ,XU Yan-feng ,YUE Xiao-qiang ,LI Min ,LU Jin-cai ,LING Chang-quan (1. Department of Pharmacognosy, School of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 3. Department of Traditional Chinese Medicine, No. 546 Hospital of PLA, Xinjiang Malan 841700,Chinas4. Department of Military Health, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433 ,China) |
| Abstract: | Objective：To optimize the preparation technology of extract of Portulaca oleracea L.. Methods： With extrac tion yield of α-linolenic acid, the yield of extract and the content of α-linolenic acid in extract as criteria,whose content was determined by HPLC method, the preparation process conditions were optimized by L9 （3^4 ） orthogonal test. Results： The best preparation technology conditions were as follows： concentrating the extract under reduced pressure till there was no ethanol, adding water to 0. 5 g/mL, adjusting pH to 7. 5-8. 0 with 10% NaOH solution, centrifugalizing for 1 min at 5 000 r/min, adding water to 1 g/mL and centrifugalizing for 10 min at 5 000 r/min. Conclusion： The best preparation technology is reliable, convenient and practical. |
| Keywords: | Portulaca oleracea L α-linolenic acid orthogonal test technology pharmaceutical |
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