ATB-FUNGUS 3及ROSCO法体外抗酵母样菌药物敏感性试验评价

目的 探讨商品化的真菌药物敏感性检测方法ATB-FUNGUS3(ATB-F3法)及ROSCO纸片扩散法(ROSCO法)与标准的CLSI M27-A2(标准MIC法)方法的符合率.方法 分别用法国生物梅里埃公司的ATB-F3法和丹麦ROSCO法及标准MIC法平行检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物对280株临床分离的假丝酵母菌的体外药物敏感性并进行比较.结果 ATB-F3法与标准MIC法检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物的符合率分别为100.0%、100.0%、91.8%、91.4%、91.4%;ROSCO法与标准MIC法的符合率分别为92.9%、98.6%、88.2%、88.9%、88.6%;ATB-F3法与ROSCO法的符合率分别92.9%、98.6%、93.2%、86.8%、89.6%.结论 ATB-F3法及ROSCO法与MIC法均有很高的符合率,而以ATB-F3法更高;两种商品化的方法间符合率较好,临床实验室均可选用,但宜选用MIC法或ATB-F3法以尽可能减少误差.

Susceptibility of Yeasts Using the ATB FUNGUS 3 and ROSCO Disk Diffusion Testing Methods:An in vitro Evaluation

OBJECTIVE To compare with the coincidence rate between the microbroth ATB-FUNGUS 3 (ATB-F3) method and ROSCO disk diffusion testing method for in vitro determination of the susceptibility of Candida spp, considering CLSI M27-A2 as the reference method. METHODS ATB-F 3 method and Rosco disk diffusion testing method and CLSI M27-A2 method were applied parallel in detecting the susceptibility of 280 strains of Candida to 5 kinds of antifungal agents and the results were compared. RESULTS The coincidence rate between ATB-F 3 method and CLSI M27-A2 method for antifungal susceptibility testing to 5-flucytosine, amphoteriein B, fluconazole, itraconazole and voriconazole was 100.0%, 100.0%, 91.8%, 91.4%, and 91.4%, respectively. Between Rosco disk diffusion testing method and CLSI M27-A2 method, the coincidence rate was 92.9%, 98.6%, 88.2%, 88.9%, and 88.6%, respectively. Between ATB-FUNGUS 3 method and Rosco disk diffusion testing method, the coincidence rate was 92.9%, 98.6%, 93.2%, 86.8%, and 89.6%, respectively. CONCLUSIONS The coincidence is good between ATB-F3 method and ROSCO disk diffusion testing method and CLSI M27-A2 method, but ATB-F 3 is better than ROSCO. We recommend ATB-F 3 method for clinical laboratories to avoid the error.