抗前列腺特异膜抗原单克隆抗体的前列腺癌免疫病理研究

目的 观察前列腺特异膜抗原（ＰＳＭ）在前列腺癌（ＰＣａ）中的表达情况，探讨其与前列腺癌病理组织学分级及与内分泌治疗效果的关系，方法 采用ＥｎＶｉｓｉｏｎ＾ＴＭ两步法免疫组织化学染色方法，用ＰＳＭ单克隆抗体Ｅｄ－５对３６例前列腺癌（Ｐｃａ）、１０例正常前列腺组织、１０例前列腺良性增生、６是性前列腺炎、３８例非前列腺肿瘤（包括膀胱癌、肾透明细胞癌、睾丸肿瘤等）进行染色。结果 ＰＳＭ在正常前列腺组织、慢

An immunohistochemical study of prostatic carcinoma with the use of monoclonal antibody against prostate specific membrane antigen

Objective To determine the expression of prostate specific membrane antigen (PSM) in prostate cancer and to investigate the relationship between the PSM expression and histologic grading of prostatic carcinoma and the hormone refractory prostate cancer. Methods 36 cases of prostate cancer, 6 prostatitis, 10 benign prostate hyperplasia (BPH), 10 normal prostate tissue and 38 non prostate neoplasms (including bladder cancer, renal cancer, testis neoplasm, colon rectal cancer,etc.) were analysed for the expression of PSM by using Ed 5 monoclonal antibody and EnVision TM immununohistochemical staining. Results Both benign and neoplastic prostate cells except 3 cases of prostate cancer were stained positively, the staining being intense in hormone refractory lesions.Non prostate neoplasms were stained negatively with the antibody.The relationship between the PSM expression and the histologic grading of prstate carcinoma was difficult to be established. Conclusions The monoclonal antibody Ed 5 was organ specific.It might be used for the imaging and the targeting treatment of prostate cancer.