槲皮素和原花青素对豚鼠白癜风模型黑素合成的影响

目的：明确槲皮素、原花青素单体对白癜风豚鼠皮肤黑素合成的影响。方法：利用过氧化氢构建38例豚鼠白癜风模型成功后，随机分为5组，分别给予乙醇（A组）、蒸馏水（B组）、0.1%8-甲氧补骨脂素（8-MOP）（C组）、0.5%槲皮素（D组）、0.1%原花青素（E组）以及0.3%原花青素（F组）外涂皮肤，其中C组用药1h后予日光照射10min。治疗期间，每2周拍摄记录豚鼠皮损色素沉着。40天后，处死全部模型并取被试区皮肤。肉眼下及HE染色观察皮肤基本病理改变；黑素颗粒染色法（Fontana-Masson法）检测含黑素颗粒细胞数量。结果：除A、B组外，其余4组在治疗期间均出现不同程度色素沉着。HE染色发现，与A、B组相比，其余4组皮肤厚度更厚，色沉较明显。Fontana-Masson法显示，与A、B组相比，其余4组黑素颗粒明显增多（P均<0.05），其中，D、F组明显多于E组（P均<0.05）。结论：槲皮素以及原花青素单体对白癜风豚鼠模型的皮肤具有复色作用。

Effect of quercetin and procyanidins on melanogenesis in the guinea pig model of vitiligo

Objective: To determine the effect of quercetin and procyanidins on melanogenesis in the guinea pig model of vitiligo. Methods: Thirty eight guinea pig models of vitiligo were successfully induced by perhydrol. The guinea pig models of vitiligo were randomly divided into 5 groups: ethanol (group A), distilled water (group B), 0.1% 8-methoxypsoralen（8-MOP）(group C), 0.5% quercetin (group D), 0.1% proanthocyanidin (group E) and 0.3% proanthocyanidin (group F). The guinea pig models in the group C were given sunlight exposure for ten minutes after taking medicine for 1h. The level of pigmentation on skin lesions in guinea pigs was recorded every 2 weeks. All the animal models were killed and the skin of the tested area was taken after 40 days. The basic pathological changes were observed by naked eyes and HE staining. The cell numbers of melanocyte granules were detected by Fontana-Masson method. Results: There were different degrees of pigmentation in group C-D during treatment. HE staining showed that the thicknesses of skin in group C-D were thicker and the pigmentation was more obvious than that in group A and B. Fontana-Masson method showed that melanin granules in group C-D were significantly more than those in group A and B (Ps < 0.05) and the numbers of melanin granules in group D and F were much more than that in group E (Ps < 0.05 ). Conclusion: Quercetin and procyanidins may increased the pigment in the guinea pig models of vitiligo.