胶原膜包裹或应用环孢素A改善鸡羽根角蛋白材料的组织相容性

目的 观察经胶原膜包裹或应用免疫抑制剂后,鸡羽根角蛋白(CCK)材料在体埋植对周围组织的影响.方法 30只SD大鼠,随机均分为5组.将经特殊处理的CCK埋植入竖脊肌组织中.实验组:A组为CCK材料埋植加术后两周腹腔内注射免疫抑制剂环孢素A(CsA,日剂量5 mg/kg),B组为CCK材料浸泡CsA(2.5 mg/ml)后埋植,C组为胶原膜包裹CCK材料后埋植,D组仅埋植CCK材料.空白对照组仅作切口,不埋植材料.分别于术后2、4、8周取材,常规切片染色,光镜下观察埋植材料的降解情况及对周围组织的影响,免疫组织化学术检测周围组织内T淋巴细胞浸润情况.结果 术后各组大鼠一般状况良好,术后第2、4、8周,可见大量巨噬细胞,尤其是多核巨细胞分布于材料边缘,吞噬材料.炎性细胞数量较少.第2、4、8周A组CD3阳性表达细胞数较B组少,与D组有显著差异(P＜0.05),A组与空白对照组无显著差异(P＞0.05).结论 短期应用免疫抑制剂可显著改善CCK的在体组织相容性;CCK在体内可降解,巨噬细胞,尤其多核巨细胞参与了该过程.

Collagen gel coating or cyclosporine A for improving histocompatibility of chicken calamus keratin

OBJECTIVE: To improve the histocompatibility of chicken calamus keratin (CCK) graft by collagen-gel coating or using of cyclosporine A (CsA). METHODS: Thirty SD rats were equally randomized into 5 groups, and in 4 of them, CCK implantation into the bilateral erector spinae was performed on different treatment protocols. In group A, the rats received daily intraperitoneal injection of CsA (5 mg/kg) for two consecutive weeks after CCK implantation; in group B, CCK was soaked in CsA (2.5 mg/ml) solution at 4 degrees Celsius; for 48 h before grafting; in group C, CCK coated with collagen gel was grafted; and in group D, only CCK was implanted. Rats in the fifth group received only cutaneous incision as well as muscular dissection to serve as the blank control. CCK degradation and its effect on the surrounding tissues were observed at 2, 4 and 8 weeks after grafting. Immunohistochemistry was performed to identify T lymphocyte infiltration in the host tissues. RESULTS: All the rats survived the operation. Numerous macrophages, especially multinucleated giant cells occurred on the peripheral of the CCK grafts, and small degraded CCK pieces were observed in their cytoplasm. Only a few inflammatory cells were seen in the host tissues. At 2, 4 and 8 weeks after CCK implantation, only a few CD3-positive cells were found in all the groups, and in group A and B, the density of T lymphocytes was significantly lower than that in group D, and there was no significant difference between group A and the blank control group. CONCLUSIONS: CsA significantly improves the histocompatibility of CCK material, and short-term systemic CsA administration achieves the best results. Macrophages, especially multinucleated giant cells participate in CCK degradation in vivo.