不同气腹介质对肿瘤细胞体外生长的影响

目的研究腹腔镜手术中不同气腹介质对肿瘤细胞体外生长的影响。方法采用3 H TdR掺入法 ,比较肿瘤细胞在经过“CO2 气腹”、“He气腹”、“Air气腹”条件后 ,肿瘤细胞摄取胸腺嘧啶核苷合成DNA(CPM值 )的情况。结果“气腹”后第 2 4hCO2 组CPM值较He组、Air组及对照组明显增高 (F =4 7 5 76 5 ,P <0 0 1) ,He组、Air组及对照组之间差异无显著性 (P >0 0 5 )。“气腹”后第 4 8h、72h、96hCO2 组较He组、Air组及对照组CPM值明显增高 (F =116 183,10 82 92 ,5 116 75 ,P <0 0 1) ,He组较Air组、对照组CPM值下降 (P <0 0 1) ,Air组与对照组之间差异无显著性 (P >0 0 5 )。结论CO2 可刺激肿瘤细胞生长 ,He可抑制肿瘤细胞生长。

The effects of different pneumoperitoneum on in vitro tumor cells growth

ObjectiveThis study was to evaluate the effects of different pneumoperitoneum used in laparoscopy on in vitro tumor cells growth. Methods 3H-TdR incorporation was used to compare the DNA synthesis (CPM value) of tumor cells after exposure to simulated laparoscopic environments, composed of carbon dioxide, helium and room air, respectively. Results At 24 hours after pneumoperitoneum, compared with group of helium, air and control, CPM increased in group of carbon dioxide (F=47.576 5,P<0.01), there was no difference among group of helium ,air and control (P>0.05). At 48,72 and 96 hours, pneumoperitoneum, compared with helium group, air group and control group, CPM increased significantly in carbon dioxide group (F=116.183,1082.92 and 5116.75, P<0.01);Compared with air group and control group, CPM value reduced in helium group(P<0.01), there was no significant difference between air group and control group (P>0.05). Conclusion Carbon dioxide promotes tumor cells growth, while helium restrains the growth of tumor cells. [