| Effect of Calmodulin and Voltage-dependent Ca~(2+) Channel on the Proliferation of Heptoma Cells Induced by Epidermal Growth Factor |
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| 引用本文： | 吴斌文,王家(马龙),袁顺玉,崔武任. Effect of Calmodulin and Voltage-dependent Ca~(2+) Channel on the Proliferation of Heptoma Cells Induced by Epidermal Growth Factor[J]. 华中科技大学学报(医学英德文版), 2003, 23(1) |
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| 作者姓名： | 吴斌文 王家(马龙) 袁顺玉 崔武任 |
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| 作者单位： | InstituteofLiverDisease，TongjiHospital，HuazhongUniversityofScienceandTechnology，InstituteofLiverDisease，TongjiHospital，HuazhongUniversityofScienceandTechnology，InstituteofLiverDisease，TongjiHospital，HuazhongUniversityofScienceandTechnology，DepartmentofNuclearMedicine，TongjiMedicalCollege，HuazhongUniversityofScienceandTechnology Wuhan430030，Wuhan430030，Wuhan430030，Wuhan430030 |
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| 摘 要： | Epidermal growth factor(EGF) has been impli-cated as a hepatotrophic factor during liver regenera-tion.EGF plays a role in the proliferation of hep-atoma cells.In general,EGF induces tumor cells di-vision and proliferation through a series of signaltransduction by binding with EGF receptor(EGFR)and activate its thyrosine protein kinase and therebyinducing phosphoration of itself and its protein sub-strate[1] .This postreceptorsingnal transduction isas-sociated with phosphoylinositolpathw…
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Effect of Calmodulin and Voltage-dependent Ca2+ Channel on the Proliferation of Heptoma Cells Induced by Epidermal Growth Factor |
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| WU Binwen,WANG Jialong,YUAN Shunyu Institute of Liver Disease,Tongji Hospital,Huazhong University of Science and Technology,Wuhan CUI Wuren. Effect of Calmodulin and Voltage-dependent Ca2+ Channel on the Proliferation of Heptoma Cells Induced by Epidermal Growth Factor[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2003, 23(1) |
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| Authors: | WU Binwen WANG Jialong YUAN Shunyu Institute of Liver Disease Tongji Hospital Huazhong University of Science Technology Wuhan CUI Wuren |
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| Affiliation: | 1. Institute of Liver Disease, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030
2. Department of Nuclear Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 |
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| Abstract: | The effect of thyrosine kinase, calmodulin and voltage-dependent Ca 2+ channel on the proliferation of hepatoma cells induced by EGF was studied. Hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured by 3H-TdR incorporation. 10 -9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (P<0.05), and this effect might be significantly inhibited by tyrosine kinase inhibitor (P<0.001). Calmodulin inhibitor W-7 had no effect on the basic phase of cultured hepatoma cells (P> 0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (P<0.001). Voltage-dependent Ca 2+ channel inhibitor Varapamil had no inhibition on the proliferation of hepatoma cells induced by EGF (P>0.05). It had no effect on the basic phase of cultured hepatoma cells (P>0.05). It is suggested that tyrosine kinase and Ca 2+-calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF, and voltage-dependent Ca 2+ channel is independent of the effect of EGF. |
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| Keywords: | epidermal growth factor human hepatoma cell line Ca 2+-calmodulin-dependent pathway tyrosine kinase voltage-dependent Ca 2+ channel |
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