| Synthesis of CCK-8 Tetrapeptide Fragment by Enzymatic Method |
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| 引用本文： | 项光亚,Heiner Eckstein. Synthesis of CCK-8 Tetrapeptide Fragment by Enzymatic Method[J]. 华中科技大学学报(医学英德文版), 2003, 23(3): 234-235. DOI: 10.1007/BF02829500 |
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| 作者姓名： | 项光亚 Heiner Eckstein |
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| 作者单位： | Pharmacy School，Institute of Organic Chemistry Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，University of Tuebingen，Tuebingen，Germany，72076 |
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| 摘 要： | Cholecystokinin (CCK )isa polypeptidehor monecontaining 33aminoacidsresiduewithgastroin testinal,biliarandbrainactivities Itiswidelydis tributedinthevertebratecentralnervoussystem ,anditcanstimulateextensivelytheliberationofinsulinandglucagonfromtheLangerhansislets GreateffortshavebeendevotedtothesynthesisofCCK 8,itsderivativesandanalogues Amongthedifferentmethods ,theenzymaticmethodisthemosteffectiveonewithmanyadvantagesoverconventionalchemicalmethodologies :enzymespecificitysuppress essid…
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| 关 键 词： | CCK-8 enzymatic method α-chymotrpsin papain |
| 收稿时间： | 2003-05-02 |
Synthesis of CCK-8 tetrapeptide fragment by enzymatic method |
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| Xiang Guangya,Heiner Eckstein. Synthesis of CCK-8 tetrapeptide fragment by enzymatic method[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2003, 23(3): 234-235. DOI: 10.1007/BF02829500 |
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| Authors: | Xiang Guangya Heiner Eckstein |
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| Affiliation: | 1. Pharmacy School,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030
2. Institute of Organic Chemistry,University of Tuebingen,Tuebingen,Germany,72076 |
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| Abstract: | Summary The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEf successively. These three steps were catalyzed by α-chymotrpsin, Papain and α-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass. XIANG Guangya, male, born in 1968, Lecturer |
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| Keywords: | CCK-8 enzymatic method α -chymotrpsin papain |
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