骨髓间充质干细胞向软骨细胞分化的研究进展

骨髓间充质干细胞(BMSCs)是存在于骨髓内的一种多能干结缔组织前体细胞,具有多向分化潜能,也是最有可能成为软骨组织工程的种子细胞来源。本文从BMSCs诱导成软骨细胞的方法和研究进展做一综述,如体外细胞团聚集诱导培养、体外单层细胞诱导培养、体外三维支架环境中诱导培养、体内软骨微环境诱导培养、和软骨细胞体外共培养诱导以及基因转染诱导培养等,这也是软骨组织工程研究中不可缺少的重要环节。

Differentiation of Bone Marrow Stem Cells into Chondrogenic Phenotype

Bone marrow stem cells(BMSCs) are pluripotent progenitor cells that exist in the bone marrow.It can be differentiated into various cell phenotypes and served as optimal autogenous cell source for cartilage tissue engineering.This review intends to give an overview on the various methods in the field of differentiation of BMSCs into chondrogenic phenotype,such as in vitro culture of cell aggregates,in vitro monolayer culture,in vitro three dimensions scaffold culture,in vivo cartilage microenvironment culture,co-culture with chondrocytes and gene transfection culture.It is a indispensable for cartilage engineering research.