γ射线照射对兔眼损伤效应的实验研究

目的 观察胰高血糖素样肽－2对放射损伤小鼠小肠肠粘膜屏障和消化吸收功能恢复的影响。方法 昆明种小鼠经8Gy^60Coγ射线全身性一次照射后随机分为放射损伤对照组和胰高血糖素样肽－2处理组，设1、3、5d3个时相点，检测肠通透性、血浆内毒素含量、葡萄糖吸收能力及肠上皮蔗糖酶和乳糖酶活性变化。结果 8Gy ^60Coγ射线照射后1、3、5d肠通透性和葡萄糖吸收能力、蔗糖酶、乳糖酶活性分别显著高于和低于正常对照组（P＜0．01或P＜0．05），血浆内毒素含量在1、3d时显著高于正常对照组（P＜0．01或P＜0．05）；胰高血糖素样肽－2处理组肠通透性、血浆内毒素含量、葡萄糖吸收能力和蔗糖酶、乳糖酶活性分别在3d和5d时恢复正常。结论 胰高血糖素样肽－2可促进放射损伤小鼠肠粘膜屏障和消化吸收功能的恢复。

Effect of glucagon-like peptide 2 on recovery of intestinal function from radiation injury in mice

Objective To investigate the effect of glucagon-like peptide 2(GLP-2) on recovery of intestinal epithelial function from radiation injury in mice. Methods Kunming mice received a single dose of 8 Gy total-body irradiation from 60 Co gamma-rays followed by treatment with GLP-2 or vehicle.Intestinal permeability,plasma endotoxin content,glucose absorption,and sucrase and lactase activities were measured on days 1,3,5 after treatment. Results In irradiated mice intestinal permeability and glucose absorption were significantly higher, and sucrase and lactase activities lower than those in normal mice on days 1,3,5(P<0^01 or P<0^05).Plasma endotoxin content in irradiated mice on days 1,3 was significantly higher than that in the normal mice (P<0^01 or P<0^05).In GLP-2-treated mice,intestinal permeability,glucose absorption,and plasma endotoxin content restored to normal on day 3 sucrase and lactase activities restored to normal on day 5. Conclusion GLP-2 can promote recovery of intestinal mucosal barrier function,digestion and absorption function from radiation injury.;