急性给予ApoE4升高大鼠皮层神经元内静息[Ca~(2+)]i

目的研究载脂蛋白E(ApoE)对大鼠皮层神经元内游离钙离子水平Ca2+]i的影响。方法用激光共聚焦显微镜(LSCM)和Fluo-3/AM荧光探针标记检测皮层神经元钙信号瞬间动态变化;用N-甲基-D-天冬氨酸(NMDA)受体阻断剂MK-801观察ApoE4对其影响。结果ApoE4可以呈时间及浓度依赖性升高神经元内静息Ca2+]i(P<0.01或P<0.05),MK-801可以部分阻断ApoE4所致的静息Ca2+]i升高(P<0.05或P<0.01);而ApoE3无影响。结论急性给予ApoE4能升高神经元内静息Ca2+]i,NMDA受体的激活可能参与了ApoE4所致的胞内钙信号改变与其神经毒作用。

Acute application of ApoE4 increases the neuronal resting [Ca~(2+)] i in rat cortex

Objective To investigate the acute effect of apolipoprotein E(apoE)on the intracellular free Ca2+ of rat cortical neurons.Methods The intracellular resting calcium level in cultured primary rat cortical neurons was measured by using confocal fluorescent imaging technique. MK-801,an N-methyl-D-aspartate (NMDA) receptor noncompetitive antagonist,was employed to test potential function of apoE4 through blocking NMDN receptor. ResultsAcute application of apoE4,but not apoE3,significantly increased the resting ...