光动力作用对人肝癌细胞HepG2的影响及其机制探讨

目的 研究血卟啉衍生物光动力作用诱导人肝癌细胞HepG2凋亡及其机制.方法 应用流式细胞仪分析光动力作用后细胞凋亡及免疫组化染色检测凋亡相关蛋白bcl-2蛋白、bax蛋白表达水平.结果 血卟啉衍生物光动力组人肝癌细胞HepG2凋亡率达(25.28±1.52)%,与对照组相比,差异有极显著意义(P＜0.01);并且光动力作用后凋亡相关蛋白bcl-2表达显著高于对照组(P＜0.05).结论 血卟啉衍生物光动力作用具有诱导人肝癌细胞HepG2凋亡的生物学效应,而凋亡调控蛋白bcl-2表达水平的降低可能是血卟啉衍生物光动力作用诱导人肝癌细胞HepG2凋亡的一个重要机制.

Effect of photodynamic therapy with hematoporphyrin on the apoptosis and bcl-2, bax expression of human hepatocellular carcinoma cells

Objective To investigate the apoptosis of human hepatocellular carcinoma cells induced by photodynamic therapy with hematoporphyrin and its mechanism. Methods The apoptosis of human hepatocellular carcinoma cell-line HepG2 was detected by flow cytometry with P1 staining and the translational levels of bcl-2 gene and bax gene by immunohistochemical staining. Results The apoptotic rate of hepatocellular carcinoma cell line HepG2 after photodynamic therapy with hematoporphyrin was significantly increased, which was higher than that in the controls(P<0.01). The expression of bcl-2 protein in hematoporphyrin group was lower than that in the controls(P<0.05). Conclusion Photodynamic therapy with hematoporphyrin might obviously trigger apoptosis of HepG2 cells by down-regulating the translational level of bcl-2 gene.