| 乙型肝炎病毒DNA与血清免疫标志物的关系分析 |
| |
| 引用本文： | 郭辉,闵娟,叶健忠.乙型肝炎病毒DNA与血清免疫标志物的关系分析[J].华中医学杂志,2007,31(3):207-208,F0003. |
| |
| 作者姓名： | 郭辉 闵娟 叶健忠 |
| |
| 作者单位： | 1. 518020,深圳,暨南大学第二临床学院
2. 518033,深圳,深圳国际旅行卫生保健中心 |
| |
| 摘 要： | 目的 探讨乙型肝炎病毒DNA(HBV DNA)与血清免疫标志物的关系.方法 荧光定量聚合酶链反应法 (FQ-PCR) 检测血清样本中HBV DNA含量;酶联免疫吸附法(ELISA)检测HBV血清标志物.结果 A组(HBsAg和HBeAg阳性)、B组(大三阳)、C组(HBsAg 、HBcAb阳性)和D组(小三阳)的HBV DNA阳性率分别为96.7%、87.6%、52.2%和38.2%,各组之间均有显著性差异(P＜0.05); A组中阳性样本的HBV DNA量显著高于其它组别(P＜0.05); 315份HBV DNA阳性标本全部携带有HBsAg;HBsAg( )HBeAg( )和HBsAg( )HBeAg(-)中HBV DNA阳性率分别为89.1%和44.0%,有显著性差异(P＜0.05).结论 HBsAg是HBV感染灵敏的血清标志物;FQ-PCR定量检测HBV DNA作为血清学方法的补充对乙肝的临床诊断具有重要意义.
|
| 关 键 词： | 乙型肝炎病毒DNA 荧光定量PCR 血清标志物 乙型肝炎 病毒 血清学方法 免疫标志 物的关系 分析 markers serum HBV DNA 意义 临床诊断 乙肝 补充 定量检测 感染 阳性标本 样本 显著性差异 阳性率 小三阳 |
| 修稿时间： | 2007-03-30 |
Relationship between HBV DNA and serum markers |
| |
| Guo Hui,Ye Jianzhong,Min Juan.Relationship between HBV DNA and serum markers[J].Central China Medical Journal,2007,31(3):207-208,F0003. |
| |
| Authors: | Guo Hui Ye Jianzhong Min Juan |
| |
| Institution: | 1 The Second Affiliated Hospital of Medical College of Jinan University, Shenzhen 518020 |
| |
| Abstract: | Objective To explore the relationship between serum HBV markers and HBV DNA in serum. Methods HBV DNA in the serum was determined by FQ-PCR and serum HBV markers tested by ELISA method.Results The level of HBV DNA in group A (HBsAg and HBeAg positive) was significantly higher than in groups B and C (P<0.05); All samples with HBV DNA( ) were also positive for HBsAg. Respectively, 89.1% and 44.0% in the group of HBsAg( )HBeAg( ) and the group of HBsAg( )HBeAg(-) were HBV DNA( ). There was significant difference between the two groups.Conclusion HBsAg is the sensitive serum marker for HBV infection;FQ-PCR has the important clinical value as a supplement of serum detected methods for diagnosis of hepatitis B. |
| |
| Keywords: | Hepatitis B virus DNA Fluorescence quantitative PCR Serum HBV markers |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
