| 人类线粒体转移方法初报 |
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| 引用本文： | 蔡炯,沈洁,王婷,鞠丽梅,刘一农.人类线粒体转移方法初报[J].实验动物与比较医学,1999(2):3. |
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| 作者姓名： | 蔡炯 沈洁 王婷 鞠丽梅 刘一农 |
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| 作者单位： | ：中国医学科学院 中国协和医科大学实验动物研究所，北京
100021 |
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| 摘 要： | 将人肝脏匀浆、差速离心、等密度梯度离心纯化得到线粒体,通过显微注射法将其转移到小鼠的受精卵细胞质内,有61 % 的卵注射后存活,而存活的卵中53 % 能顺利完成第一次卵裂。提示将人类线粒体向实验动物小鼠转移具有可行性
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| 关 键 词： | 线粒体 显微注射 人 |
Preliminary Study on Methods of Human Mitochondria Transfer |
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| CAI Jiong,SHEN Jie,WANG Ting,JU Li-Mei,LIU Yi-Nong.Preliminary Study on Methods of Human Mitochondria Transfer[J].Laboratory Animal and Comparative Medicine,1999(2):3. |
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| Authors: | CAI Jiong SHEN Jie WANG Ting JU Li-Mei LIU Yi-Nong |
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| Institution: | CAI Jiong, SHEN Jie, WANG Ting, JU
Li-Mei, LIU Yi-Nong;
(Institute of Laboratory Animal Science, CAMS, Beijing 100021, China) |
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| Abstract: | The human liver tissue was homogenized. It''s mitochondria were separated and purified by fraction centrifuge and step ingredient centrifuge. The purified mitochondria were micro-injected into the cytoplasm of the feritlized eggs. There is sixty-one percent microinjected eggs survived immediately after the manipulation. Among them, fifty-three percent eggs pass the primary division. The results suggested the possibility of production of mice bring human mitochondria. |
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| Keywords: | Mitochondria Micro-injection Human |
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