神经酰胺诱导小鼠皮层神经元凋亡

本文采用改进的体外生物测定法分析恒河猴月经周期中促卵泡刺激素（ＦＳＨ）、促黄体生成素（ＬＨ）的变化情况。结果表明：雌猴的ＦＳＨ峰出现在月经周期的第１０２±１０天，峰值：５７２±４８ｎｇ／ｍｌ；ＬＨ峰出现在月经周期的第１２０±１６天，峰值：９６３±９７ｎｇ／ｍｌ，且为滤泡期、黄体期平均值的７～８倍。证实了在雌性恒河猴的月经周期中，其ＬＨ分泌高峰与ＦＳＨ分泌高峰不在同一时间出现的现象。

Ceramide Induces Apoptosis in Culture Cortical Neurons of Fetal Mice

In this study, the effect of natural ceramide has been tested on primary cultures of cortical neurons. In order to study if the cortical neurons undergoing apoptosis, some markers such as the leakage rate of lactate dehydrogenase(LDH) and the MTT metabolic rate were assessed, also the morphological changes were observed by Giemsa staining. DNA ladders were determined by gel electrophoresis, as well as the cell ultrastructurales were observed under electronmicroscope. The results showed that 500 to 1000nM of ceramide could induce apoptosis of culture cortical neurons in dose-dependent and time-dependent way after 12 hrs of incubation. The ultrastructural changes are consistent with the changes observed by Giemsa staining or DNA ladders on gel electrophoresis.