人工繁殖恒河猴的血清前清蛋白、转铁蛋白、苹果酸脱氢酶和醇脱氢酶遗传多态性研究

用薄层聚丙烯酰胺凝胶电泳方法分析118头人繁殖恒河猴血清四种蛋白质和同工酶遗传基因位点的多态性,结果表明,除醇脱氢酶(ADH)为单态外,其余三个基因位点均表现多态,前清蛋白(PA)可分为AA、AB、AC、AD和BB、CC,EE七种表型,各基因的频率为A 0.85,B 0.072,C 0.042,D 0.009,E 0.034转铁蛋白(Tf)可分为CC、DD、EE、FFGG、CD、CE、CG、CH、DE、DF、DG、DH、EF、EG、EH、FG十七种表型,墓因频率为C 0.064,D 0.380,E 0.188,F 0.111,G 0.244,H 0.014,苹果酸脱氢酶(MDH)可分MDH)1-1和MDH2-1两种表型,基因频率为MDH~10.958和MDH~20.042。

Study of Genetic Polymorphism of Plasma Prealbumin, Transferrin, MDH and ADH from Laboratory-Bred Rhesus Monkeys

Genetic polymorphism of two plasma proteins prealbumin ( PA ) and transferrin ( Tf ) , and two plasma isozymes, malate dehydrogenase ( MDH) and alcohol dehydrogenase ( ADH ) from 118 laboratory-bred Rhesus monkeys ( M.mulatta ) was analysed by thin-layer polyacry lamide gel electrophoresis.The result suggested that ADH showed the monomorphism, the PA.Tf and MDH showed the polymorphism.The electrophoric pattern of PA can be divided into 7 phenotyp s, that is AA, AB, AC, AD and BB, CC. EE. The genes frequencies of A, B, C, D and E were 0.343, 0.072, 0.042, 0.009 and 0.081 respectively .The Tf can be divided into 17 phenotypes, that is CC, DD, EE, FF, GG, CD, CE, CG, CH, DE, DF, DG, EF, EG, EH, FG.The genes frequencies of C, D, E, F, G and H were 0.064, 0.38, 0.133, 0.111, 0.244, and 0.014 respectively. The MDH isozyme can be divided into MDH1-1 and MDH2-1 two phenotypes MDH1 and MDH2 frequencies were 0.953 and 0.042. The experimen tal results were compared to those of previously reported experiments.