ASGP受体介导的体内基因转移

我们针对肝细胞特异的ＡＳＧＰ（去唾液酸糖蛋白）受体，构建了一种具有肝细胞特异导向性的基因转移载体系统，该载体包括两种共价结合的功能成分：一种为ＡＳＧＰ，作为配体，与肝细胞表面特异的ＡＳＧＰ受体结合；另一种为多聚左旋赖氨酸（ｐｏｌｙ－Ｌ－ｌｙｓｉｎｅ），与ＤＮＡ以强静电作用相结合。小鼠尾静脉注射３２Ｐ－ＤＮＡ－ｐｏｌｙ－Ｌ－ｌｙｓｉｎｅ－ＡＳＯＲ（去唾液酸ａｌ酸性糖蛋白）或等量的３２Ｐ－ＤＮＡ、ＡＳＯＲ、ｐｏｌｙ－ＤＬ－ｌｙｓｉｎｅ单体混合物，２０小时后，小鼠肝、脾、肾各组织的放射性计数结果表明该载体系统具有较强的肝组织特异性。

ASGP Receptor Mediated Gene Transfer in Vivo

ehave developed a gene transfer system for targeting DNA tohepatocytes in vivo using a DNA carrier that takes advantage of ASGP(asiQio-glycoprotein) receptors for hepatocytes. This carrier systemincludes two kinds of funct ional components: one is ASGP whichcan bind ASGP receptors, the other is poly-L-lysine which can bindDNA in a strong electric interaction. Intravenous injection of 32P-DNA-poly-L-lysine-ASOR (asio lo-orosomucoid) or cqual amount of the mix-ture of individual const ituents demonstrated more speciffc target ingin the liver than in the spleen and the kidneys.