表皮生长因子治疗兔角膜碱烧伤研究

本试验制成兔角膜碱烧伤模型，应用重组表皮生长因子（ｒｈＥＧＦ）滴眼剂对碱烧伤后的兔角膜溃疡创面进行治疗。６３只纯种新西兰白兔分为７组，每组９只，其中５组为治疗组，另２组为对照组，治疗组分别用每毫升０．５，５，２０，５０和１００μｇ表皮生长因子滴眼剂滴眼，对照组分别用纤维结合蛋白和氯霉素滴眼。伤后２４，４８，７２，９６及１２０ｈ裂隙灯荧光素染色，照像观察溃疡面积，经计算机图像处理，计算。结果显示５组治疗组创面愈合时间明显短于对照组，（Ｐ＜０．０５）。提示ＥＧＦ对角膜碱烧伤后的溃疡面愈合有促进作用。

Experiment Study of EGF on Alkali Burned Corneal

To determine the effect of EGF(epidermal growth factor)on alkali burned corneal wound healing of rabbit,sixty three New Zealand rabbits alkali burned corneal wounds were produced,Five groups (n=9)wound eyes were divided and treated with EGF solution (0.5,5,20,50,100ng/ml) and the other groups (n=9) treated with FN and Chloromicitine solution as controls.The presence of an epithelial defect was assessed by staining with fluoescein dye.The corneal wounds were measured with slit-lamp after corneal wound operations (24,48,72,96,120hr).All data were analyzed by compute test.The EGF treated groups showed faster corneal healing than the controls groups (P<0.05).The results suggested that the effect of EGF accelerate the healing an alkali burned corneal.