高、低转移性小鼠前胃鳞癌细胞株的建立及其部分特性的观察

为了比较研究转移性癌细胞的特性，通过克隆技术及体内移植，从具有自发血道转移能力的小鼠前胃鳞癌细胞系（ＭＦＣ）中分离和建成了两株具有高转移和低转移能力的小鼠前胃鳞癌细胞株ＭＦＣ－Ｃ３４和ＭＦＣ－Ｃ６４。高转移的ＭＦＣ－Ｃ３４细胞生长快，细胞倍增时间为２５．２ｈ，平均分裂指数达到７２．２５％，给动物皮下移植后肿瘤生长快，肺转移率达５２．９４％；低转移的ＭＦＣ－Ｃ６４细胞倍增时间为６３．６ｈ，平均分裂指数为４１．３３％，给动物皮下移植后肿瘤生长慢，肺转移率仅为１５．６２％。高低转移性鳞癌细胞株的建立，为比较研究转移性鳞癌的特性提供了两个细胞株有用的材料和工具。

Establishment of two Mouse Forestomach SquamousCarcinoma Cell Strains with High and LowMetastatic Capacities and Comparative Observationof their Characteristics

o study and comparison the characteristics of high and low met-astatic cancer cells, two quamous carcinoma cell strains derived fromthe mouse forestornach carcinoina cell line (MFC) being possessedspontaneous hemotogenous metastasis by clonal technique and transplan-tation experinient have been established. Some comparative studies bet-ween the cells of MFC-C34 and MFC-C64 on the doubling time. mitoticindex, growth speed of tumor transplantation of the cloned cells inmice,the incidence of lung metastasis, the electrophoretic mobility ofliving cells were carried out. Shorter doubling time, higher averagemitotic index,faster growth speed and bigger volume for the tumor formedby homeotransplantation, higher incidence of lung metastasis, fasterelectrophoretic mobility were seen in the AIFC-C34, with significantlystatistical difference as compared with MFC-C64, a low metastaic capa-city cell strain.MFC-C34 and MFC-C64 cell strains will be very useful materials forthe study of the characteristics of metastatic cancer cells and experi-mental treatment of the metastasis.